Abstract
Silicon (Si) is important quasi-essential element, important for growth and productivity in plants by abetting abiotic and biotic stresses. In the recent times intensive cultivation in India has led to depletion of available Si in soils leads stagnation in the crop productivity. In this study, out of 88 rhizobacterial isolates, 24 potential isolates having significant silica solubilizing capability and exhibited plant growth-promoting characteristics were characterized at biochemical and molecular level and further to study their effect on plant growth stimulation and augment the absorption and accumulation of active silica in plants. In qualitative method, all 24 SiS-RB isolates were able to form clear zone of silica solubilization with the solubilizing index (SSI) in the range of 1.05–3.40 cm, whereas in quantitative silica solubilization the solubilized silica was observed in a range of 1.29–43.29 ppm. The 24 SiS-RB isolates further demonstrated plant growth promoting activities. Subsequently, these isolates were evaluated for their capacity to solubilize various minerals, including biotite, calc silicate, feldspar, muscovite, orthoclase, and quartzite, revealing that only six isolates had significant solubilization ability. The six potent isolates viz. SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24 showed a considerable enhancement in maize plant development under in vitro conditions, including improved antioxidant properties such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) activities. All 24 SiS-RB were subsequently analyzed for genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) analysis, and findings revealed that considerable higher genetic diversity exists among SiS-RB isolates. The integrated dendrogram exhibited similarity indices between 0.11 and 0.90, with a mean of 0.51. All potent silica-solubilizing plant growth-promoting rhizobacterial isolates were identified using 16S rDNA sequencing and belongs to Enterobacter sp., Serratia surfactantfaciens , and Klebsiella sp. These influential isolates would significantly enhance silicate management through Si based biofertilizer development for plant growth promotion under Si deficient soils.
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Claims 13
Claim · model-written summary
All 24 SiS-RB isolates formed silica solubilization clear zones with indices of 1.05–3.40 cm.
Not reportedDirection
Verbatim
e0“In qualitative method, all 24 SiS-RB isolates were able to form clear zone of silica solubilization with the solubilizing index (SSI) in the range of 1.05–3.40 cm”
Claim · model-written summary
Quantitative solubilized silica ranged from 1.29–43.29 ppm.
Not reportedDirection
Verbatim
e0“whereas in quantitative silica solubilization the solubilized silica was observed in a range of 1.29–43.29 ppm.”
e1“all 24 SiS-RB isolates were able to form clear zone of silica solubilization”
Claim · model-written summary
The 24 SiS-RB isolates demonstrated plant growth promoting activities.
Verbatim
e0“The 24 SiS-RB isolates further demonstrated plant growth promoting activities.”
Claim · model-written summary
Only six isolates significantly solubilized the tested minerals.
Verbatim
e0“Subsequently, these isolates were evaluated for their capacity to solubilize various minerals, including biotite, calc silicate, feldspar, muscovite, orthoclase, and quartzite, revealing that only six isolates had significant solubilization ability.”
e1“The 24 SiS-RB isolates further demonstrated plant growth promoting activities.”
Claim · model-written summary
The six potent isolates considerably enhanced maize development in vitro.
- Treatment
- SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24e0
- Interactor
- SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24e0symbiont
- Outcome
- plant developmente0
- Direction
- enhancemente0
- Setting
- in vitro conditionse0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The six potent isolates viz. SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24 showed a considerable enhancement in maize plant development under in vitro conditions”
Claim · model-written summary
The potent isolates improved maize catalase, superoxide dismutase, and peroxidase activities.
- Treatment
- six potent isolatese0
- Interactor
- SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24e0symbiont
- Gene
- catalase (CAT), superoxide dismutase (SOD), peroxidase (POD)e0
- Outcome
- antioxidant propertiese0
- Direction
- improvede0
- Setting
- in vitro conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The six potent isolates viz. SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24 showed a considerable enhancement in maize plant development under in vitro conditions, including improved antioxidant properties such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) activities.”
Claim · model-written summary
The potent isolates improved maize polyphenol oxidase and phenylalanine ammonia lyase activities.
- Treatment
- six potent isolatese0
- Interactor
- SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24e0symbiont
- Gene
- polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL)e0
- Outcome
- activitiese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The six potent isolates viz. SSB-2, SSB-8, SSB-11, SSB-12, SSB-21, and SSB-24 showed a considerable enhancement in maize plant development under in vitro conditions, including improved antioxidant properties such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) activities.”
Claim · model-written summary
ARDRA revealed considerable genetic diversity among SiS-RB isolates.
Verbatim
e0“All 24 SiS-RB were subsequently analyzed for genetic diversity using amplified ribosomal DNA restriction analysis (ARDRA) analysis, and findings revealed that considerable higher genetic diversity exists among SiS-RB isolates.”
Claim · model-written summary
The bacterial dendrogram had similarity indices from 0.11 to 0.90, averaging 0.51.
Not reportedDirection
Verbatim
e0“The integrated dendrogram exhibited similarity indices between 0.11 and 0.90, with a mean of 0.51.”
e1“considerable higher genetic diversity exists among SiS-RB isolates”
Claim · model-written summary
Sequencing identified potent isolates as Enterobacter sp.
- Taxon
- Enterobacter sp.
Enterobactere0 - Outcome
- identified using 16S rDNA sequencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All potent silica-solubilizing plant growth-promoting rhizobacterial isolates were identified using 16S rDNA sequencing and belongs to Enterobacter sp., Serratia surfactantfaciens , and Klebsiella sp.”
Claim · model-written summary
Sequencing identified potent isolates as Serratia surfactantfaciens.
- Taxon
- Serratia surfactantfaciensSerratia surfactantfaciense0
- Outcome
- identified using 16S rDNA sequencinge0
Verbatim
e0“All potent silica-solubilizing plant growth-promoting rhizobacterial isolates were identified using 16S rDNA sequencing and belongs to Enterobacter sp., Serratia surfactantfaciens , and Klebsiella sp.”
Claim · model-written summary
Sequencing identified potent isolates as Klebsiella sp.
- Taxon
- Klebsiella sp.Klebsiellae0
- Outcome
- identified using 16S rDNA sequencinge0
Verbatim
e0“All potent silica-solubilizing plant growth-promoting rhizobacterial isolates were identified using 16S rDNA sequencing and belongs to Enterobacter sp., Serratia surfactantfaciens , and Klebsiella sp.”
Claim · model-written summary
The authors propose the isolates for Si biofertilizer development to promote plants in Si deficient soils.
Verbatim
e0“These influential isolates would significantly enhance silicate management through Si based biofertilizer development for plant growth promotion under Si deficient soils.”