Abstract
Light-emitting diodes (LEDs) have historically been the standard for supplemental lighting in industrial hemp (Cannabis sativa) cultivation in a greenhouse, but there is a lack of scientific understanding in C. sativa regarding different media under various LEDs in vitro conditions. The aim of this study was to determine the top-performing LEDs and nutrient media for micropropagation of C. sativa. In this study, two different spectra (white and red LEDs) and different media [MS, B5, MS+B5, ½ MS, ½ B5, ½ MS + ½ B5, and control (without medium)] were used, maintaining constant light photoperiod. The diversity of light spectra and nutrient media showed statistically significant differences for the growth parameters of C. sativa. Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm. Also, nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media. Among the plant growth parameters, the number of shoots and nodes per plant is of great importance for clonal propagation. B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87. Each LED source required the respective medium adjustment for the best results of the desired parameters. In this respect, the study may be advantageous for the selection of the appropriate nutrient medium and led spectrum in micropropagation of C. sativa.
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Claims 7
Claim · model-written summary
Light spectra and media significantly affected growth parameters.
- Taxon
- C. sativa
Cannabis sativae0 - Treatment
- light spectra and nutrient mediae0
- Outcome
- growth parameterse0
- Direction
- differencese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The diversity of light spectra and nutrient media showed statistically significant differences for the growth parameters of C. sativa.”
Claim · model-written summary
Red LED produced shoot length of 7.37 cm.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- Red LEDe0
- Outcome
- shoot lengthe0
- Direction
- extraordinarily highe0
- Value
- 7.37 cme0
- Comparator
- white LEDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm.”
t0“Cannabis sativa”
Claim · model-written summary
White LED gave highest root length of 6.57 cm.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- white LEDe0
- Outcome
- root lengthe0
- Direction
- higheste0
- Value
- 6.57 cme0
- Comparator
- Red LEDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red LED provided an extraordinarily high shoot length of 7.37 cm, whereas white LED exhibited the highest root length with 6.57 cm.”
t0“Cannabis sativa”
Claim · model-written summary
Full-strength media promoted shoot growth.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- full-strength mediae0
- Outcome
- shoot growthe0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media.”
t0“Cannabis sativa”
Claim · model-written summary
Half-strength media promoted root growth.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- half-strength mediae0
- Outcome
- root growthe0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nutrient media were found to be effective on shoot growth in full-strength media, while on root growth in half-strength media.”
t0“Cannabis sativa”
Claim · model-written summary
B5 medium under red LED gave 4.64 shoots.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- B5 medium under red LEDe0
- Outcome
- shoot numbere0
- Direction
- extraordinarily highe0
- Value
- 4.64e0
- Comparator
- MS medium under white LEDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87.”
t0“Cannabis sativa”
Claim · model-written summary
MS medium under white LED gave highest node number, 5.87.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- MS medium under white LEDe0
- Outcome
- node numbere0
- Direction
- higheste0
- Value
- 5.87e0
- Comparator
- B5 medium under red LEDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5 medium under red LED provided an extraordinarily high shoot number with 4.64, whereas MS medium under white LED exhibited the highest node number with 5.87.”
t0“Cannabis sativa”
The same questions in other papers
light · growth · Cannabis sativaPositive and negative results
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- no effect
- no effect
- positive
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See all 13 papers →in vitro · growth · Cannabis sativaPositive and negative results
10 papers in the same direction as this paper · 3 in the opposite direction
- positive
- no effect
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.