Abstract
Trehalose is a nonreducing disaccharide critical for cellular integrity and stress adaptation in plants, and its synthesis relies on trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Despite their established roles in abiotic stress responses across model plants, these gene families remain underexplored in ornamental species like Petunia hybrida. Here, TPS and TPP genes in two wild petunia progenitors, P. axillaris and P. inflata, underwent a genome-wide analysis, with 10 TPS and 8–9 TPP genes being identified in each species. According to phylogenetic analysis, petunia TPS proteins cluster into two clades, while TPP proteins were classified into three clades, showing closer evolutionary ties to tomato homologs. Cis-acting elements profiling identified hormone- and stress-responsive regulatory elements (e.g., ABRE, TC-rich repeats). Expression analysis under drought, heat, and salt stress revealed dynamic temporal regulation. For instance, PaTPS4/PaTPS9 were early responders (peak at 6 h) under drought and salt stress, while PaTPS8 exhibited sustained upregulation during salt treatment. Heat stress uniquely suppressed PaTPS1,2 and PaTPP1, contrasting with broad upregulation of other members. Notably, PaTPP3 displayed delayed induction under heat. These findings underscore the functional diversity within TPS/TPP families, with specific members governing stress-specific responses. This study provides a foundational resource for leveraging these genes to enhance stress resilience and ornamental value in petunia.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Genome-wide analysis identified 10 TPS and 8-9 TPP genes in each of the wild petunia progenitors P. axillaris and P. inflata.
Verbatim
e0“TPS and TPP genes in two wild petunia progenitors, P. axillaris and P. inflata, underwent a genome-wide analysis, with 10 TPS and 8–9 TPP genes being identified in each species”
Claim · model-written summary
Petunia TPS proteins cluster into two clades in phylogenetic analysis.
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=60){kind=small}
PetuniaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
_flowers_in_Westgate_Gardens%2C_Canterbury.jpg?width=500)
Verbatim
e0“petunia TPS proteins cluster into two clades”
Claim · model-written summary
Petunia TPP proteins were classified into three clades and showed closer evolutionary ties to tomato homologs.
- Gene
- TPP proteinse0
- Outcome
- classified into three cladese0
- Value
- three cladese0
- Comparator
- tomato homologse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TPP proteins were classified into three clades, showing closer evolutionary ties to tomato homologs”
t0“Petunia”
Claim · model-written summary
Cis-acting element profiling identified hormone- and stress-responsive regulatory elements in petunia TPS/TPP genes.
- Outcome
- hormone- and stress-responsive regulatory elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cis-acting elements profiling identified hormone- and stress-responsive regulatory elements”
t0“Petunia”
Claim · model-written summary
PaTPS4 and PaTPS9 were early responders under drought and salt stress, peaking at 6 h.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PaTPS4/PaTPS9 were early responders (peak at 6 h) under drought and salt stress”
t0“Petunia”
Claim · model-written summary
PaTPS8 showed sustained upregulation during salt treatment.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PaTPS8 exhibited sustained upregulation during salt treatment”
t0“Petunia”
Claim · model-written summary
Heat stress uniquely suppressed PaTPS1,2 and PaTPP1, in contrast to broad upregulation of other members.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat stress uniquely suppressed PaTPS1,2 and PaTPP1, contrasting with broad upregulation of other members”
t0“Petunia”
Claim · model-written summary
PaTPP3 displayed delayed induction under heat.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PaTPP3 displayed delayed induction under heat”
t0“Petunia”