Abstract
Background. The maintenance of purity, control of identity, and reproduction of seeds of different taxonomic groups of plants entering the collection requires simple and rapid screening methods that allow preliminary identification and registration of the material. The most suitable method for these purposes is SDS-electrophoresis of seed storage proteins, but its variants known from the literature mainly include analysis of the total protein pattern, which is usually very heterogeneous and thus complicates the interpretation of the obtained results. The objective of the work was to find a clearly identifiable and species-specific group of polypeptides of a particular protein in this multicomponent pattern. Materials and methods. The objects of the study were seed proteins of 47 accessions of five species of Vicia L. subgenus Vicia , analyzed by SDS-electrophoresis in 12.5% PAAG. Results and discussion. A comparison of patterns of the total extracted seed proteins separated by electrophoresis in the presence and absence of 2-mercaptoethanol identified a group of the 11S globulin (legumin) basic polypeptides, which allows visual unambiguous determination of a species (both self- and cross-pollinated) to which vetch seeds belong. It was found that the accessions differed only in the intensity of individual bands within the species, but not in the band composition, indicating the species-specific pattern of the 11S globulin basic polypeptides. Clear differences in the basic polypeptides composition were observed between the closely related species of the V. narbonensis complex, which are difficult to distinguish in the field. The species specificity of the patterns in this group of polypeptides correlates with the reproductive isolation of the species. In turn, cultivated and weedy accessions of Pannonian vetch, V. pannonica Crantz, of different geographical origins from the VIR collection with a likely predominance of cross-pollination showed homogeneity in the patterns of the legumin basic polypeptides. The patterns of the 11S globulin basic polypeptides of V. hyaeniscyamus Mout., V. narbonensis L., V. serratifolia Jacq., V. johannis Tamamschjan in Karyagin, and V. pannonica were recorded using the “soybean scale” in the form of a “polypeptide formula”. Conclusion . Therefore, the proposed method, including the identification of the 11S globulin basic polypeptides in the multicomponent pattern of seed proteins, can be recommended for rapid screening of seed collections of both self-pollinated and cross-pollinated species of the genus Vicia . This simple method is also promising for identifying species of other dicotyledonous crops.
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Claims 8
Claim · model-written summary
The 11S globulin basic polypeptides allow unambiguous visual identification of vetch seed species.
Verbatim
e0“identified a group of the 11S globulin (legumin) basic polypeptides, which allows visual unambiguous determination of a species (both self- and cross-pollinated) to which vetch seeds belong”
t0“Vicia L.”
Claim · model-written summary
Within Vicia species, accessions varied in band intensity but shared band composition.
Verbatim
e0“the accessions differed only in the intensity of individual bands within the species, but not in the band composition”
t0“Vicia L.”
Claim · model-written summary
Closely related species in the V. narbonensis complex differed clearly in basic polypeptide composition.
- Taxon
- V. narbonensis complex
Vicia narbonensise0 - Outcome
- basic polypeptides compositione0
- Comparator
- closely related species of the V. narbonensis complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Clear differences in the basic polypeptides composition were observed between the closely related species of the V. narbonensis complex”
t0“Vicia L.”
Claim · model-written summary
Species-specific polypeptide patterns correlate with reproductive isolation in Vicia.
- Taxon
- Vicia L.t0
- Organ / stage
- reproductive isolation of the speciese0
- Outcome
- species specificity of the patternse0
Not reportedDirection
Verbatim
e0“The species specificity of the patterns in this group of polypeptides correlates with the reproductive isolation of the species”
t0“Vicia L.”
Claim · model-written summary
Cultivated and weedy V. pannonica accessions from different origins had homogeneous legumin basic polypeptide patterns.
- Taxon
- V. pannonica Crantz
Vicia pannonicae0 - Material
- cultivated and weedy accessions of Pannonian vetche0
- Outcome
- homogeneity in the patterns of the legumin basic polypeptidese0
- Setting
- VIR collectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cultivated and weedy accessions of Pannonian vetch, V. pannonica Crantz, of different geographical origins from the VIR collection with a likely predominance of cross-pollination showed homogeneity in the patterns of the legumin basic polypeptides”
Claim · model-written summary
Basic polypeptide patterns for five Vicia species were recorded as polypeptide formulas.
- Taxon
- V. hyaeniscyamus Mout.Vicia hyaeniscyamuse0
- Outcome
- “polypeptide formula”e0
- Value
- 11S globulin basic polypeptidese0
Verbatim
e0“The patterns of the 11S globulin basic polypeptides of V. hyaeniscyamus Mout., V. narbonensis L., V. serratifolia Jacq., V. johannis Tamamschjan in Karyagin, and V. pannonica were recorded using the “soybean scale” in the form of a “polypeptide formula””
Claim · model-written summary
The authors recommend the polypeptide method for rapid screening of Vicia seed collections.
- Outcome
- rapid screening of seed collectionse0
- Qualifier
- both self-pollinated and cross-pollinated speciese0
ViciaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proposed method, including the identification of the 11S globulin basic polypeptides in the multicomponent pattern of seed proteins, can be recommended for rapid screening of seed collections of both self-pollinated and cross-pollinated species of the genus Vicia”
Claim · model-written summary
The authors suggest the method could identify species of other dicotyledonous crops.
Verbatim
e0“This simple method is also promising for identifying species of other dicotyledonous crops”