Abstract
Light quality significantly influences plant growth and development by interacting with photoreceptors, leading to reversible and irreversible outcomes. This study provides novel insights into the species-specific effects of light-emitting diodes (LEDs) on the morphological characteristics and genetic stability of Eurycoma longifolia hairy root cultures (ELHRCs) under different light qualities. LED treatments included white (400 nm–700 nm), blue (440 nm), mint green (530 nm), red (660 nm) and a combination of blue with red (1:1) (440 nm + 660 nm) applied for 8, 10 and 12 weeks. Morphological changes and growth were assessed alongside genetic stability through direct amplification of minisatellite DNA regions (DAMD) and inter-simple sequence repeat (ISSR) markers analysis using 12 primers. The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots. Additionally, the study demonstrated how spectral quality influences hairy roots growth and morphology. The high percentage of genetic similarity highlights LEDs as a promising tool for in vitro culture of ELHRCs. These findings represent the first comprehensive report on the combined effects of LED spectral quality on growth, morphological changes and genetic stability in ELHRCs.
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Claims 7
Claim · model-written summary
DAMD genetic similarity was 90.6% at 8 weeks under LED treatments.
- Taxon
- Eurycoma longifolia
Eurycoma longifoliat0 - Organ / stage
- hairy rootse0
- Outcome
- genetic similaritye0
- Value
- 90.6%e0
- Qualifier
- under all LED treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots.”
t0“Eurycoma longifolia”
Claim · model-written summary
DAMD genetic similarity was 100% at 10 and 12 weeks under LED treatments.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Organ / stage
- hairy rootse0
- Outcome
- genetic similaritye0
- Value
- 100%e0
- Qualifier
- under all LED treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots.”
t0“Eurycoma longifolia”
Claim · model-written summary
ISSR genetic similarity was 100% at 8 weeks under LED treatments.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Organ / stage
- hairy rootse0
- Outcome
- genetic similaritye0
- Value
- 100%e0
- Qualifier
- under all LED treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots.”
t0“Eurycoma longifolia”
Claim · model-written summary
ISSR genetic similarity was 98.2% at 10 weeks under LED treatments.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Organ / stage
- hairy rootse0
- Outcome
- genetic similaritye0
- Value
- 98.2%e0
- Qualifier
- under all LED treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots.”
t0“Eurycoma longifolia”
Claim · model-written summary
ISSR genetic similarity was 90.3% at 12 weeks under LED treatments.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Organ / stage
- hairy rootse0
- Outcome
- genetic similaritye0
- Value
- 90.3%e0
- Qualifier
- under all LED treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results showed genetic similarity of 90.6% after 8 weeks and 100% after 10 and 12 weeks (DAMD) and 100%, 98.2% and 90.3% after 8, 10 and 12 weeks, respectively (ISSR) under all LED treatments, confirming the genetic stability of the hairy roots.”
t0“Eurycoma longifolia”
Claim · model-written summary
LED spectral quality influenced hairy root growth and morphology.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Organ / stage
- hairy rootse0
- Treatment
- spectral qualitye0
- Outcome
- growth and morphologye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, the study demonstrated how spectral quality influences hairy roots growth and morphology.”
t0“Eurycoma longifolia”
Claim · model-written summary
The authors consider LEDs promising for in vitro culture of these hairy roots.
- Taxon
- Eurycoma longifoliaEurycoma longifoliat0
- Outcome
- promising tool for in vitro culture of ELHRCse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The high percentage of genetic similarity highlights LEDs as a promising tool for in vitro culture of ELHRCs.”
t0“Eurycoma longifolia”