Abstract
In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meristems were isolated from actively growing shoots of stock plants and transferred to Driver and Kuniyuki Walnut (DKW) culture medium containing either 0.0, 0.5, 1.0, 2.0, or 5.0 μM meta-Topolin to study their shoot proliferation response. Resulting shoot cultures were transferred to medium containing varying levels of Indole Acetic Acid (IAA), Indole Butyric Acid (IBA), or Naphthalene Acetic Acid (NAA), solely or in combination, and were subjected to a 10-day dark incubation followed by a 16 h/8 h light/dark period to identify the best treatment for root production. Among the different shoot proliferation treatments studied, the maximum number of shoots was produced on the control medium that was devoid of any meta-Topolin. Cultures grown on medium containing 5.0 μM meta-Topolin exhibited hyperhydricity, where shoots appeared translucent and pale green in color; were characterized by water-soaked lesions; and leaves appeared curled and brittle in contrast to healthy looking cultures. Among the various rooting treatments studied, shoots grown in the dark for 10 days exhibited the highest frequency of rooting on medium containing 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAA. Full developed plants with a robust shoot and root system were transferred to soil, acclimatized under conditions for high humidity, and then transferred to ambient conditions in 4 weeks. The micropropagation protocol developed here allows for rapid multiplication of disease-free plants in C. sativa cultivars.
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Claims 5
Claim · model-written summary
The control medium without meta-Topolin produced the maximum number of shoots.
- Taxon
- Cannabis sativa
Cannabis sativae0 - Treatment
- meta-Topoline1
- Outcome
- number of shootse1
- Direction
- maximume1
- Comparator
- control mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cannabis sativa L. cultivars”
e1“Among the different shoot proliferation treatments studied, the maximum number of shoots was produced on the control medium that was devoid of any meta-Topolin”
Claim · model-written summary
Cultures on 5.0 μM meta-Topolin exhibited hyperhydricity.
- Taxon
- Cannabis sativaCannabis sativae0
- Treatment
- 5.0 μM meta-Topoline2
- Outcome
- hyperhydricitye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cannabis sativa L. cultivars”
e2“Cultures grown on medium containing 5.0 μM meta-Topolin exhibited hyperhydricity, where shoots appeared translucent and pale green in color”
Claim · model-written summary
The highest rooting frequency occurred on 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAA after 10 days in the dark.
- Taxon
- Cannabis sativaCannabis sativae0
- Treatment
- 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAAe3
- Outcome
- frequency of rootinge3
- Direction
- higheste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cannabis sativa L. cultivars”
e3“shoots grown in the dark for 10 days exhibited the highest frequency of rooting on medium containing 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAA”
Claim · model-written summary
Fully developed plants with a robust shoot and root system were transferred to soil.
- Taxon
- Cannabis sativaCannabis sativae0
- Outcome
- robust shoot and root systeme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cannabis sativa L. cultivars”
e4“Full developed plants with a robust shoot and root system were transferred to soil”
Claim · model-written summary
The protocol allows rapid multiplication of disease-free Cannabis sativa plants.
- Taxon
- Cannabis sativaCannabis sativae0
- Outcome
- rapid multiplication of disease-free plantse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cannabis sativa L. cultivars”
e5“The micropropagation protocol developed here allows for rapid multiplication of disease-free plants in C. sativa cultivars”
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