Paper · 2025 · Asian Journal of Science Technology Engineering and Art

Bioinsecticidal Activities of Azadirachta indica A. Juss (Meliaceae) Seed Oil Extracts on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae) on Stored Maize

Obembe Olusola Michael; Philip-Attah Mary Tejumade; Omotoso Olumuyiwa Temitope

15
claims
60
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by-nc-sa

Abstract

The preservation of stored grains against weevil infestations is critical for ensuring food security and minimizing post-harvest losses in Nigeria, where insect pests remain a major constraint to effective grain storage. This study evaluates the efficacy of seed extracts of Azadirachta indica in controlling Sitophilus zeamais. Seeds were extracted using five solvents—methanol, ethanol, acetone, petroleum ether, and n-hexane—and the resulting oils were tested for their effects on insect mortality, oviposition, adult emergence, grain damage, and long-term storage viability. Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence, with complete suppression observed in ethanol-, petroleum ether-, and n-hexane-treated grains. These treatments also fully prevented grain damage and weight loss for three months, while methanol and acetone extracts allowed limited damage (9.19% and 8.18%, respectively). Germination tests showed high viability (95.25–98.25%) across all treatments, with the lowest germination recorded in petroleum ether extract (95.25%). The findings suggest that A. indica seed extracts are highly effective in managing S. zeamais infestations and represent a viable alternative to synthetic insecticides for safe and sustainable grain storage.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-sa. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 15

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanol extract caused 100% weevil mortality within 96 hours.

Taxon
Stored Maizet0
Treatment
ethanol extracte0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
mortalitye0e1
Direction
most effectivee0
Value
100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure”

✓ located verbatim in the source · abstract, characters 601–692 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

N-hexane extract caused 88.25% weevil mortality.

Taxon
Stored Maizet0
Treatment
n-hexanee0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
mortalitye0e1
Value
88.25%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%)”

✓ located verbatim in the source · abstract, characters 694–790 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Petroleum ether extract caused 85.75% weevil mortality.

Taxon
Stored Maizet0
Treatment
petroleum ethere0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
mortalitye0e1
Value
85.75%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%)”

✓ located verbatim in the source · abstract, characters 694–790 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Methanol extract caused 82.25% weevil mortality.

Taxon
Stored Maizet0
Treatment
methanole0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
mortalitye0e1
Value
82.25%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%)”

✓ located verbatim in the source · abstract, characters 694–790 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Acetone extract caused 76.15% weevil mortality.

Taxon
Stored Maizet0
Treatment
acetonee0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
mortalitye0e1
Value
76.15%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%)”

✓ located verbatim in the source · abstract, characters 694–790 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The extracts significantly reduced weevil oviposition and adult emergence.

Taxon
Stored Maizet0
Treatment
Extractse0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
oviposition and adult emergencee0e1
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 792–859 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanol treated grains completely suppressed oviposition and adult emergence.

Taxon
Stored Maizet0
Treatment
ethanol-e0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
oviposition and adult emergencee0e1
Direction
complete suppressione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“complete suppression observed in ethanol-, petroleum ether-, and n-hexane-treated grains”

✓ located verbatim in the source · abstract, characters 866–954 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Petroleum ether treated grains completely suppressed oviposition and adult emergence.

Taxon
Stored Maizet0
Treatment
petroleum ether-e0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
oviposition and adult emergencee0e1
Direction
complete suppressione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“complete suppression observed in ethanol-, petroleum ether-, and n-hexane-treated grains”

✓ located verbatim in the source · abstract, characters 866–954 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

N-hexane treated grains completely suppressed oviposition and adult emergence.

Taxon
Stored Maizet0
Treatment
n-hexane-treated grainse0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
oviposition and adult emergencee0e1
Direction
complete suppressione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“complete suppression observed in ethanol-, petroleum ether-, and n-hexane-treated grains”

✓ located verbatim in the source · abstract, characters 866–954 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanol, petroleum ether and n-hexane treatments prevented grain damage and weight loss for three months.

Taxon
Stored Maizet0
Treatment
These treatmentse0
Interactor
Sitophilus zeamaisSitophilus zeamaist1peste0
Outcome
grain damage and weight losse0
Direction
fully preventede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“These treatments also fully prevented grain damage and weight loss for three months”

✓ located verbatim in the source · abstract, characters 956–1039 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Methanol treated grains had 9.19% damage.

Taxon
Stored Maizet0
Treatment
methanole0
Outcome
damagee0
Value
9.19%e0

Verbatim

e0

“methanol and acetone extracts allowed limited damage (9.19% and 8.18%, respectively)”

✓ located verbatim in the source · abstract, characters 1047–1131 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Acetone treated grains had 8.18% damage.

Taxon
Stored Maizet0
Treatment
acetonee0
Outcome
damagee0
Value
8.18%e0

Verbatim

e0

“methanol and acetone extracts allowed limited damage (9.19% and 8.18%, respectively)”

✓ located verbatim in the source · abstract, characters 1047–1131 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Germination viability remained 95.25–98.25% across treatments.

Taxon
Stored Maizet0
Outcome
viabilitye0
Value
95.25–98.25%e0

Verbatim

e0

“Germination tests showed high viability (95.25–98.25%) across all treatments”

✓ located verbatim in the source · abstract, characters 1133–1209 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Petroleum ether extract had the lowest germination viability, 95.25%.

Taxon
Stored Maizet0
Treatment
petroleum ether extracte0
Outcome
germinatione0
Direction
loweste0
Value
95.25%e0

Verbatim

e0

“lowest germination recorded in petroleum ether extract (95.25%)”

✓ located verbatim in the source · abstract, characters 1220–1283 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors regard neem extracts as a viable alternative to synthetic insecticides for grain storage.

Taxon
Stored Maizet0
Interactor
S. zeamaisSitophilus zeamaise0pest
Outcome
viable alternative to synthetic insecticidese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Sitophilus zeamais
Sitophilus zeamais · InteractorThis image was released by the Agricultural Research Service , the research agency of the United States Department of Agriculture , with the ID k3980-17 (next) · Public domain · Wikimedia Commons ↗

Verbatim

e0

“A. indica seed extracts are highly effective in managing S. zeamais infestations and represent a viable alternative to synthetic insecticides for safe and sustainable grain storage”

✓ located verbatim in the source · abstract, characters 1311–1491 · show in text ↑
t0

“Stored Maize”

✓ located verbatim in the source · title, characters 158–170 · show in text ↑
t1

“Sitophilus zeamais”

✓ located verbatim in the source · title, characters 90–108 · show in text ↑
e1

“Results indicate that ethanol extract was the most effective, inducing 100% mortality within 96 hours of exposure, followed by n-hexane (88.25%), petroleum ether (85.75%), methanol (82.25%), and acetone (76.15%). Extracts also significantly reduced oviposition and adult emergence”

✓ located verbatim in the source · abstract, characters 579–859 · show in text ↑

ethanol · Sitophilus zeamais · Zea mays (maize)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

2 in the opposite direction

  1. negative
  2. no effect
See all 3 papers →

acetone · Sitophilus zeamais · Zea mays (maize)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive
See all 4 papers →

methanol · Sitophilus zeamais · Zea mays (maize)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

ether · Sitophilus zeamais · Zea mays (maize)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

hexane · Sitophilus zeamais · Zea mays (maize)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

See all 7 papers →

botanical · Sitophilus zeamais · Zea mays (maize)Positive and negative results

117 papers: 113 positive, 4 negative or no effect

112 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. negative
  3. no effect

113 papers more in this group

See all 117 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.