Abstract
Seed oil and protein contents are critical agronomic traits that determine soybean quality. However, the key loci and corresponding genes controlling these quality traits remain to be elucidated. Here, we performed bulked segregant analysis by sequencing (BSA-seq) using an F4 population derived from a cross between the cultivars Heinong 35 (HN35) and Dengke 3 (DK3). A major soybean oil and protein quantitative trait locus (QTL) designated as q-OP18 was identified on chromosome 18, and the sugar transporter gene GmSWEET46 was further cloned. Haplotype analysis revealed that a single-nucleotide polymorphism (SNP) in the sixth exon of GmSWEET46 results in an amino acid change between HN35 and DK3 and is associated with seed oil and protein content, suggesting its important role in determining seed quality in soybean. GmSWEET46 is expressed during the early stages of seed and pod development and localizes to the plasma membrane, indicating its potential function as a sugar transporter. Further studies demonstrated that GmSWEET46 can regulate seed protein content, oil content, and seed size in Arabidopsis and soybean. Collectively, this study provides a novel locus and gene for regulating soybean seed traits and offers valuable resources for the breeding of high-quality and high-yielding soybean cultivars.
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Claims 6
Claim · model-written summary
A major soybean oil and protein QTL, q-OP18, was identified on chromosome 18.
- Taxon
- soybean
Glycine maxe0 - Gene
- q-OP18e0
- Outcome
- quantitative trait locus (QTL) designated as q-OP18 was identified on chromosome 18e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A major soybean oil and protein quantitative trait locus (QTL) designated as q-OP18 was identified on chromosome 18”
Claim · model-written summary
The sugar transporter gene GmSWEET46 was cloned.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmSWEET46e0
- Outcome
- gene GmSWEET46 was further clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sugar transporter gene GmSWEET46 was further cloned”
t0“Soybean”
Claim · model-written summary
An exon-six SNP in GmSWEET46 changes amino acid between cultivars and associates with oil and protein content.
- Taxon
- SoybeanGlycine maxt0
- Material
- HN35 and DK3e0
- Gene
- GmSWEET46e0
- Outcome
- seed oil and protein contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a single-nucleotide polymorphism (SNP) in the sixth exon of GmSWEET46 results in an amino acid change between HN35 and DK3 and is associated with seed oil and protein content”
t0“Soybean”
Claim · model-written summary
GmSWEET46 is expressed early in seed and pod development.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- early stages of seed and pod developmente0
- Gene
- GmSWEET46e0
- Outcome
- expressed during the early stages of seed and pod developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmSWEET46 is expressed during the early stages of seed and pod development”
t0“Soybean”
Claim · model-written summary
GmSWEET46 localizes to the plasma membrane.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmSWEET46t0
- Outcome
- localizes to the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“localizes to the plasma membrane”
t0“GmSWEET46 Regulates Seed Oil and Protein Content in Soybean”
Claim · model-written summary
GmSWEET46 regulates protein and oil content and seed size in Arabidopsis and soybean.
- Taxon
- Arabidopsis and soybean
ArabidopsisGlycine maxe0 - Gene
- GmSWEET46e0
- Outcome
- seed protein content, oil content, and seed sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmSWEET46 can regulate seed protein content, oil content, and seed size in Arabidopsis and soybean”