Abstract
BACKGROUND: Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc). While resistant cultivars like 'Khai Pra Ta Bong' (AAA genome) have been identified, the genetic basis of resistance remains unclear. This study uses RNA sequencing (RNA-seq) to investigate gene expression changes in 'Khai Pra Ta Bong' over seven days post-infection with Rsc isolate MY4101. Candidate genes associated with resistance were also evaluated in other cultivars, including resistant, moderately resistant, and highly susceptible cultivars. RESULTS: RNA-seq analysis identified several genes associated with BBD resistance mechanisms, showing significant upregulation as early as 12 h post-inoculation. Key molecular processes, including xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteins, were enriched at 24 h post-inoculation, highlighting the activation of effector-triggered immunity (ETI). Quantitative real-time RT-PCR (qRT-PCR) validation further confirmed the differential expression of several key defense-related genes in other banana cultivars. CONCLUSIONS: The study highlights the potential of these differentially expressed genes as genetic markers for BBD resistance, which could be valuable for breeding programs aimed at enhancing resistance in banana cultivars. These findings provide important insights into the molecular mechanisms of disease resistance and contribute to developing more efficient strategies for sustainable banana production and global food security.
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Claims 5
Claim · model-written summary
Several genes associated with resistance to banana blood disease were significantly upregulated as early as 12 h after Rsc inoculation in Khai Pra Ta Bong.
- Taxon
- Bananase1
- Material
- Khai Pra Ta Bonge6
- Treatment
- post-infection with Rsc isolate MY4101e6
- Interactor
- Ralstonia syzygii subsp. celebesensisRalstonia syzygii subsp. celebesensise1pathogen
- Outcome
- several genes associated with BBD resistance mechanismse2
- Direction
- upregulatione2
- Qualifier
- as early as 12 h post-inoculatione2
Not reportedComparator
Verbatim
e1“Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc)”
e2“RNA-seq analysis identified several genes associated with BBD resistance mechanisms, showing significant upregulation as early as 12 h post-inoculation”
e6“gene expression changes in 'Khai Pra Ta Bong' over seven days post-infection with Rsc isolate MY4101”
Claim · model-written summary
Key molecular processes were enriched at 24 h after inoculation with Rsc in banana.
- Taxon
- Bananase1
- Treatment
- post-infection with Rsc isolate MY4101e6
- Interactor
- Ralstonia syzygii subsp. celebesensisRalstonia syzygii subsp. celebesensise1pathogen
- Gene
- xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteinse3
- Outcome
- Key molecular processese3
- Direction
- enrichede3
- Qualifier
- at 24 h post-inoculatione3
Not reportedComparator
Verbatim
e1“Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc)”
e3“Key molecular processes, including xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteins, were enriched at 24 h post-inoculation, highlighting the activation of effector-triggered immunity (ETI)”
e6“gene expression changes in 'Khai Pra Ta Bong' over seven days post-infection with Rsc isolate MY4101”
Claim · model-written summary
Activation of effector-triggered immunity is highlighted as the mechanism behind the enriched processes.
Verbatim
e1“Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc)”
e3“Key molecular processes, including xyloglucan endotransglucosylase hydrolases, receptor-like kinases, and glycine-rich proteins, were enriched at 24 h post-inoculation, highlighting the activation of effector-triggered immunity (ETI)”
Claim · model-written summary
qRT-PCR confirmed differential expression of several key defense-related genes in other banana cultivars.
Verbatim
e4“Quantitative real-time RT-PCR (qRT-PCR) validation further confirmed the differential expression of several key defense-related genes in other banana cultivars”
Claim · model-written summary
The differentially expressed genes have potential as genetic markers for banana blood disease resistance in breeding.
Verbatim
e1“Bananas are a globally significant crop, but their production is threatened by banana blood disease (BBD), caused by Ralstonia syzygii subsp. celebesensis (Rsc)”
e5“The study highlights the potential of these differentially expressed genes as genetic markers for BBD resistance”