Abstract
ABSTRACT Curcumin content and expression patterns of curcumin synthase ( CURS ) genes in three tissues (corms, leaves and inflorescences) of 16 Curcuma alismatifolia genotypes were analysed in this study. This study aimed to investigate the variability of curcumin content and the expression patterns of CURS genes in different tissues of various C. alismatifolia genotypes, in order to identify potential resources for curcumin extraction and genetic improvement. The curcumin synthase 2 ( CURS2 ) and curcumin synthase 3 ( CURS3 ) gene sequences were successfully cloned. Significant variations in curcumin content were observed among different genotypes and tissues. In corms, Twister (0.299 mg · g −1 ) and Doitung (0.296 mg · g −1 ) had the highest curcumin levels. In inflorescences, Lanna Snow (0.375 mg · g −1 ) had the highest curcumin levels. In leaves, NewSolo (0.783 mg · g −1 ) had the highest curcumin levels. Quantitative PCR analysis revealed tissue-specific expression patterns of CURS genes: curcumin synthase 1 ( CURS1 ) was highly expressed in corms, CURS2 in leaves and CURS3 in inflorescences. Additionally, CURS2 and CURS3 were successfully cloned from C. alismatifolia . Both genes have a CDS length of 1173 bp, encoding 390 amino acids, with high sequence conservation. Phylogenetic analysis indicated close evolutionary relationships between CURS genes and those from Curcuma xanthorrhiza, Curcuma longa and Zingiber officinale . This study provides a basis for curcumin extraction and genetic improvement of C. alismatifolia , and contributes to further research on curcumin biosynthesis.
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Claims 13
Claim · model-written summary
CURS2 and CURS3 gene sequences were cloned from Curcuma alismatifolia.
- Taxon
- Curcuma alismatifolia
Curcuma alismatifoliat0 - Gene
- CURS2e0
- Outcome
- gene sequences were successfully clonede0
- Value
- CURS3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The curcumin synthase 2 ( CURS2 ) and curcumin synthase 3 ( CURS3 ) gene sequences were successfully cloned”
t0“Curcuma alismatifolia”
Claim · model-written summary
Curcumin content varied significantly among genotypes and tissues.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Outcome
- curcumin contente0
- Direction
- variationse0
- Qualifier
- different genotypes and tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant variations in curcumin content were observed among different genotypes and tissues”
t0“Curcuma alismatifolia”
Claim · model-written summary
Twister corms had one of the highest curcumin levels, 0.299 mg per gram.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Material
- Twistere0
- Organ / stage
- cormse0
- Outcome
- curcumin levelse0
- Direction
- higheste0
- Value
- 0.299 mg · g −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In corms, Twister (0.299 mg · g −1 ) and Doitung (0.296 mg · g −1 ) had the highest curcumin levels”
t0“Curcuma alismatifolia”
Claim · model-written summary
Doitung corms had one of the highest curcumin levels, 0.296 mg per gram.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Material
- Doitunge0
- Organ / stage
- cormse0
- Outcome
- curcumin levelse0
- Direction
- higheste0
- Value
- 0.296 mg · g −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In corms, Twister (0.299 mg · g −1 ) and Doitung (0.296 mg · g −1 ) had the highest curcumin levels”
t0“Curcuma alismatifolia”
Claim · model-written summary
Lanna Snow inflorescences had the highest curcumin levels, 0.375 mg per gram.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Material
- Lanna Snowe0
- Organ / stage
- inflorescencese0
- Outcome
- curcumin levelse0
- Direction
- higheste0
- Value
- 0.375 mg · g −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In inflorescences, Lanna Snow (0.375 mg · g −1 ) had the highest curcumin levels”
t0“Curcuma alismatifolia”
Claim · model-written summary
NewSolo leaves had the highest curcumin levels, 0.783 mg per gram.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Material
- NewSoloe0
- Organ / stage
- leavese0
- Outcome
- curcumin levelse0
- Direction
- higheste0
- Value
- 0.783 mg · g −1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, NewSolo (0.783 mg · g −1 ) had the highest curcumin levels”
t0“Curcuma alismatifolia”
Claim · model-written summary
CURS1 expression was high in corms.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Organ / stage
- cormse0
- Gene
- CURS1e0
- Outcome
- highly expressede0
- Direction
- highlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“curcumin synthase 1 ( CURS1 ) was highly expressed in corms”
t0“Curcuma alismatifolia”
Claim · model-written summary
CURS2 expression was high in leaves.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Organ / stage
- leavese0
- Gene
- CURS2e0
- Outcome
- tissue-specific expression patternse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CURS2 in leaves”
e1“Quantitative PCR analysis revealed tissue-specific expression patterns of CURS genes: curcumin synthase 1 ( CURS1 ) was highly expressed in corms, CURS2 in leaves and CURS3 in inflorescences”
t0“Curcuma alismatifolia”
Claim · model-written summary
CURS3 expression was high in inflorescences.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Organ / stage
- inflorescencese0
- Gene
- CURS3e0
- Outcome
- tissue-specific expression patternse1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CURS3 in inflorescences”
e1“tissue-specific expression patterns of CURS genes”
t0“Curcuma alismatifolia”
Claim · model-written summary
CURS2 and CURS3 each had a 1173 bp coding sequence.
- Taxon
- C. alismatifoliaCurcuma alismatifoliae1
- Gene
- CURS2 and CURS3e1
- Outcome
- CDS lengthe0
- Value
- 1173 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both genes have a CDS length of 1173 bp”
e1“CURS2 and CURS3 were successfully cloned from C. alismatifolia”
Claim · model-written summary
CURS2 and CURS3 each encode 390 amino acids.
- Taxon
- C. alismatifoliaCurcuma alismatifoliae1
- Gene
- CURS2 and CURS3e1
- Outcome
- encodinge0
- Value
- 390 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both genes have a CDS length of 1173 bp, encoding 390 amino acids”
e1“CURS2 and CURS3 were successfully cloned from C. alismatifolia”
Claim · model-written summary
CURS2 and CURS3 had high sequence conservation.
- Taxon
- C. alismatifoliaCurcuma alismatifoliae1
- Gene
- CURS2 and CURS3e1
- Outcome
- sequence conservatione0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with high sequence conservation”
e1“CURS2 and CURS3 were successfully cloned from C. alismatifolia”
Claim · model-written summary
Phylogenetic analysis found close relationships between CURS genes and those of three other species.
- Taxon
- Curcuma alismatifoliaCurcuma alismatifoliat0
- Gene
- CURS genese0
- Outcome
- close evolutionary relationshipse0
- Qualifier
- Curcuma xanthorrhiza, Curcuma longa and Zingiber officinalee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis indicated close evolutionary relationships between CURS genes and those from Curcuma xanthorrhiza, Curcuma longa and Zingiber officinale”
t0“Curcuma alismatifolia”