Abstract
Chaenomeles speciosa, a plant species with high ornamental value, exhibited significant darkening of its petal coloration under drought conditions. This chromatic alteration primarily stems from anthocyanin biosynthesis and transport. Through integrated proteomic and transcriptomic analyses, we identified CsABCG6, a transporter of the ABCG subfamily, as a key regulator. The molecular docking analysis preliminarily demonstrated that CsABCG6 bound to anthocyanin monomers. Functional characterization revealed a dual role of CsABCG6 in regard to anthocyanin metabolism; it displayed anthocyanin transport activity in yeast systems, whereas transient transformation assays confirmed its capacity to enhance anthocyanin biosynthesis. Stable transformation experiments in tobacco further validated the anthocyanin-promoting function of the previously identified transcription factor CsMYB6. Yeast one-hybrid (Y1H) and dual-luciferase assays (LUC) established that CsMYB6 transcriptionally activates the CsABCG6 promoter, thus collectively defining the CsMYB6-CsABCG6 signaling module as a critical mechanism for the precise dynamic regulation of anthocyanin metabolism under drought stress.
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Claims 7
Claim · model-written summary
Chaenomeles speciosa, a plant species with high ornamental value, exhibited significant darkening of its petal coloration under drought conditions.
- Taxon
- Chaenomeles speciosa
Chaenomeles speciosat0 - Treatment
- drought conditionse0
- Outcome
- petal coloratione0
- Direction
- darkeninge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Chaenomeles speciosa, a plant species with high ornamental value, exhibited significant darkening of its petal coloration under drought conditions.”
Claim · model-written summary
Through integrated proteomic and transcriptomic analyses, we identified CsABCG6, a transporter of the ABCG subfamily, as a key regulator.
- Taxon
- Chaenomeles speciosaChaenomeles speciosat0
- Gene
- CsABCG6e0
- Outcome
- CsABCG6, a transporter of the ABCG subfamily, as a key regulatore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Through integrated proteomic and transcriptomic analyses, we identified CsABCG6, a transporter of the ABCG subfamily, as a key regulator.”
Claim · model-written summary
The molecular docking analysis preliminarily demonstrated that CsABCG6 bound to anthocyanin monomers.
- Taxon
- Chaenomeles speciosaChaenomeles speciosat0
- Gene
- CsABCG6e0
- Outcome
- CsABCG6 bound to anthocyanin monomerse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“The molecular docking analysis preliminarily demonstrated that CsABCG6 bound to anthocyanin monomers.”
Claim · model-written summary
Functional characterization revealed a dual role of CsABCG6 in regard to anthocyanin metabolism; it displayed anthocyanin transport activity in yeast systems, whereas transient transformation assays confirmed its capacity to enhance anthocyanin biosynthesis.
- Taxon
- Chaenomeles speciosaChaenomeles speciosat0
- Gene
- CsABCG6e0
- Outcome
- anthocyanin transport activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Functional characterization revealed a dual role of CsABCG6 in regard to anthocyanin metabolism; it displayed anthocyanin transport activity in yeast systems, whereas transient transformation assays confirmed its capacity to enhance anthocyanin biosynthesis.”
Claim · model-written summary
Functional characterization revealed a dual role of CsABCG6 in regard to anthocyanin metabolism; it displayed anthocyanin transport activity in yeast systems, whereas transient transformation assays confirmed its capacity to enhance anthocyanin biosynthesis.
- Taxon
- Chaenomeles speciosaChaenomeles speciosat0
- Gene
- CsABCG6e0
- Outcome
- anthocyanin biosynthesise0
- Direction
- enhancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Functional characterization revealed a dual role of CsABCG6 in regard to anthocyanin metabolism; it displayed anthocyanin transport activity in yeast systems, whereas transient transformation assays confirmed its capacity to enhance anthocyanin biosynthesis.”
Claim · model-written summary
Stable transformation experiments in tobacco further validated the anthocyanin-promoting function of the previously identified transcription factor CsMYB6.
- Taxon
- tobacco
Nicotiana tabacume0 - Gene
- CsMYB6e0
- Outcome
- anthocyanin-promoting functione0
- Direction
- anthocyanin-promotinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Stable transformation experiments in tobacco further validated the anthocyanin-promoting function of the previously identified transcription factor CsMYB6.”
Claim · model-written summary
Yeast one-hybrid (Y1H) and dual-luciferase assays (LUC) established that CsMYB6 transcriptionally activates the CsABCG6 promoter, thus collectively defining the CsMYB6-CsABCG6 signaling module as a critical mechanism for the precise dynamic regulation of anthocyanin metabolism under drought stress.
- Taxon
- Chaenomeles speciosaChaenomeles speciosat0
- Treatment
- drought stresse0
- Gene
- CsMYB6e0
- Outcome
- CsABCG6 promotere0
- Direction
- activatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Chaenomeles speciosa”
e0“Yeast one-hybrid (Y1H) and dual-luciferase assays (LUC) established that CsMYB6 transcriptionally activates the CsABCG6 promoter, thus collectively defining the CsMYB6-CsABCG6 signaling module as a critical mechanism for the precise dynamic regulation of anthocyanin metabolism under drought stress.”