Abstract
Bundle sheath protoplasts (BSP) were isolated and purified from four C4 species of the phosphoenolpyruvate (PEP) carboxykinase type (Panicum maximum, P. texanum, Chloris gayana and Eriochloa borumensis), and cell organellses were separated from the BSP extract by differential centrifugation or sucrose density gradient centrifugation. Separation of the organelles was ascertained by the distribution of marker enzymes for chloroplasts, mitochondria, peroxisomes and cytoplasm. Contrary to the previous report [Rathnam and Edwards (1975) Arch. Biochem. Biophys. 171: 214], the distribution of PEP carboxykinase in BSP of P. maximum was the same as that of UDP-glucose pyrophosphorylase, a marker for cytoplasm, and PEP carboxykinase activity was not recovered in the intact chloroplasts. The same results were obtained with P. texanum, C. gayana and E. borumensis. Therefore, we conclude that PEP carboxykinase is exclusively localized in the cytoplasm of bundle sheath cells of C4 plants.
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Claims 5
Claim · model-written summary
PEP carboxykinase in P. maximum bundle sheath protoplasts had the same distribution as the cytoplasm marker UDP-glucose pyrophosphorylase.
- Taxon
- P. maximum
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Megathyrsus maximuse0 - Organ / stage
- BSPe0
- Gene
- PEP carboxykinasee0
- Outcome
- distribution of PEP carboxykinasee0
- Comparator
- UDP-glucose pyrophosphorylasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the distribution of PEP carboxykinase in BSP of P. maximum was the same as that of UDP-glucose pyrophosphorylase, a marker for cytoplasm, and PEP carboxykinase activity was not recovered in the intact chloroplasts.”
Claim · model-written summary
PEP carboxykinase activity was not recovered in intact P. maximum chloroplasts.
- Taxon
- P. maximumMegathyrsus maximuse0
- Organ / stage
- intact chloroplastse0
- Gene
- PEP carboxykinasee0
- Outcome
- PEP carboxykinase activity was not recovered in the intact chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the distribution of PEP carboxykinase in BSP of P. maximum was the same as that of UDP-glucose pyrophosphorylase, a marker for cytoplasm, and PEP carboxykinase activity was not recovered in the intact chloroplasts.”
Claim · model-written summary
P. texanum had the same cytoplasmic PEP carboxykinase localization and lack of intact-chloroplast activity.
- Taxon
- P. texanum
Urochloa texanae0 - Gene
- PEP carboxykinasee1
- Outcome
- same resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The same results were obtained with P. texanum, C. gayana and E. borumensis.”
e1“PEP carboxykinase activity was not recovered in the intact chloroplasts”
Claim · model-written summary
C. gayana had the same cytoplasmic PEP carboxykinase localization and lack of intact-chloroplast activity.
- Taxon
- C. gayana
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Chloris gayanae0 - Gene
- PEP carboxykinasee1
- Outcome
- same resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
%2C_Mexico.jpg?width=500)
Verbatim
e0“The same results were obtained with P. texanum, C. gayana and E. borumensis.”
e1“PEP carboxykinase activity was not recovered in the intact chloroplasts”
Claim · model-written summary
E. borumensis had the same cytoplasmic PEP carboxykinase localization and lack of intact-chloroplast activity.
Verbatim
e0“The same results were obtained with P. texanum, C. gayana and E. borumensis.”
e1“PEP carboxykinase activity was not recovered in the intact chloroplasts”