Paper · 2025 · DOAJ (DOAJ: Directory of Open Access Journals)

Thuja occidentalis Essential Oil: in vitro and in situ Antibacterial Potential against Phytopathogenic Bacteria and Insecticidal Activity

Miroslava Kačániová; Ján Kollár; Oleg Paulen

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verbatim quotes
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citations
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Open access · cc-by-sa

Abstract

Thuja occidentalis essential oil (TOEO) was evaluated for antibacterial activity against phytopathogenic bacteria in vitro (disc diffusion) and in situ (fruit/vegetable models), and for insecticidal activity against Megabruchidius dorsalis. Tested bacteria were Xanthomonas arboricola, Pectobacterium carotovorum, Pseudomonas syringae and Agrobacterium radiobacter. In vitro, the largest inhibition zone was observed for X. arboricola (11.67 mm), while the smallest was for A. radiobacter (7.67 mm). In situ, the strongest effect occurred at 62.5 μg/mL on strawberry against carotovorum (78.96% inhibition) and on beetroot against A. radiobacter (79.56% inhibition). Insecticidal tests showed increasing mortality of M. dorsalis with rising TOEO concentration, reaching 100% at 100%. Within the tested ranges and models, TOEO exhibited measurable antibacterial and insecticidal effects. Findings are limited to the employed matrices, concentrations and incubation conditions, and do not imply broader applicability without additional sensory/toxicity and shelf-life testing.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Thuja essential oil produced the largest inhibition zone against X. arboricola.

Taxon
Thuja occidentalisThuja occidentalist0
Treatment
essential oilt0
Interactor
X. arboricolaXanthomonas arboricolae0pathogen
Outcome
inhibition zonee0
Direction
largeste0
Value
11.67 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Thuja occidentalis
Thuja occidentalis · TaxonDaniel Dumais · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In vitro, the largest inhibition zone was observed for X. arboricola (11.67 mm), while the smallest was for A. radiobacter (7.67 mm).”

✓ located verbatim in the source · abstract, characters 366–499 · show in text ↑
t0

“Thuja occidentalis Essential Oil”

✓ located verbatim in the source · title, characters 0–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Thuja essential oil produced the smallest inhibition zone against A. radiobacter.

Taxon
Thuja occidentalisThuja occidentalist0
Treatment
essential oilt0
Interactor
A. radiobacterAgrobacterium radiobactere0pathogen
Outcome
inhibition zonee0
Direction
smalleste0
Value
7.67 mme0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Thuja occidentalis
Thuja occidentalis · TaxonDaniel Dumais · CC BY-SA 4.0 · Wikimedia Commons ↗
Agrobacterium radiobacter
Agrobacterium radiobacter · Interactor · picture from its Wikimedia Commons categoryRobert L. Anderson, USDA Forest Service, Bugwood.org · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“In vitro, the largest inhibition zone was observed for X. arboricola (11.67 mm), while the smallest was for A. radiobacter (7.67 mm).”

✓ located verbatim in the source · abstract, characters 366–499 · show in text ↑
t0

“Thuja occidentalis Essential Oil”

✓ located verbatim in the source · title, characters 0–32 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oil inhibited carotovorum on strawberry at 62.5 μg/mL.

Taxon
strawberryFragaria ×ananassae0
Treatment
62.5 μg/mLe0
Interactor
carotovorume0pathogen
Outcome
inhibitione0
Direction
strongeste0
Value
78.96%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Fragaria x ananassa
Fragaria ×ananassa · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In situ, the strongest effect occurred at 62.5 μg/mL on strawberry against carotovorum (78.96% inhibition) and on beetroot against A. radiobacter (79.56% inhibition).”

✓ located verbatim in the source · abstract, characters 500–666 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oil inhibited A. radiobacter on beetroot at 62.5 μg/mL.

Taxon
beetroote0
Treatment
62.5 μg/mLe0
Interactor
A. radiobacterAgrobacterium radiobactere0pathogen
Outcome
inhibitione0
Direction
strongeste0
Value
79.56%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Agrobacterium radiobacter
Agrobacterium radiobacter · Interactor · picture from its Wikimedia Commons categoryRobert L. Anderson, USDA Forest Service, Bugwood.org · CC BY 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“In situ, the strongest effect occurred at 62.5 μg/mL on strawberry against carotovorum (78.96% inhibition) and on beetroot against A. radiobacter (79.56% inhibition).”

✓ located verbatim in the source · abstract, characters 500–666 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

M. dorsalis mortality rose with TOEO concentration, reaching 100% at 100%.

Taxon
Thuja occidentalisThuja occidentalist0
Treatment
TOEO concentratione0
Interactor
M. dorsalisMegabruchidius dorsalise0pest
Outcome
mortality of M. dorsalise0
Direction
increasinge0
Value
100%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Thuja occidentalis
Thuja occidentalis · TaxonDaniel Dumais · CC BY-SA 4.0 · Wikimedia Commons ↗
Megabruchidius dorsalis
Megabruchidius dorsalis · InteractorSlimguy · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Insecticidal tests showed increasing mortality of M. dorsalis with rising TOEO concentration, reaching 100% at 100%.”

✓ located verbatim in the source · abstract, characters 667–783 · show in text ↑
t0

“Thuja occidentalis”

✓ located verbatim in the source · title, characters 0–18 · show in text ↑