Paper · 2025 · Journal of Advances in Biology & Biotechnology

Biochemical Diversity and Genetic Variability among Walnut (Juglans regia L.) Genotypes from Jammu and Kashmir, India

Sehar Farooq Lone

10
claims
21
verbatim quotes
0
citations
4
research areas
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Open access

Abstract

Walnut (Juglans regia L.) is cultivated primarily for its edible kernel, which serves as a valuable source of energy, essential fatty acids, proteins, carbohydrates, minerals, and bioactive compounds. Considerable variation in walnut kernel composition has been reported globally, influenced by genotype and growing environment. The present study evaluated the biochemical and nutritional composition of twenty-one walnut (Juglans regia L.) genotypes collected from diverse agro-climatic regions of Baramulla, Jammu and Kashmir, India. The investigation aimed to quantify genetic variability, heritability, and genetic advance for key kernel quality traits such as moisture, protein, ash, carbohydrate, oil, fatty acids, and flavonoid content. Moisture content ranged from 2.19 % to 3.25 %, whereas kernel oil content exhibited the greatest variability (50.39 %–70.55 %), with linoleic acid predominating (44.65 %–62.49 %). Protein content varied between 17.12 % and 20.88 %, and carbohydrate content between 8.48 % and 24.43 %. Ash content ranged from 1.12 % to 2.21 %, while total flavonoid content varied from 5.43 to 26.66 mg g⁻¹ QE. High heritability and expected genetic gain for oil, ash, carbohydrate, and flavonoid content highlight their suitability for selection-based improvement. Principal component analysis indicated that oil, linoleic acid, and flavonoid content contributed most to total variability among genotypes. The results demonstrate substantial biochemical diversity that can be utilized to develop nutritionally rich, industrially valuable walnut cultivars and support the sustainable production of high-quality genotypes in temperate regions, particularly Jammu and Kashmir.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Walnut kernel moisture ranged from 2.19 % to 3.25 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Organ / stage
kernele2
Outcome
Moisture contente0
Value
2.19 % to 3.25 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Moisture content ranged from 2.19 % to 3.25 %”

✓ located verbatim in the source · abstract, characters 744–789 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
e2

“kernel quality traits”

✓ located verbatim in the source · abstract, characters 635–656 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Walnut kernel oil content showed the greatest variation, from 50.39 % to 70.55 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Organ / stage
kernele0
Outcome
kernel oil contente0
Direction
greatest variabilitye0
Value
50.39 %–70.55 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“kernel oil content exhibited the greatest variability (50.39 %–70.55 %)”

✓ located verbatim in the source · abstract, characters 799–870 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Linoleic acid predominated in walnut kernel oil at 44.65 % to 62.49 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
linoleic acide0
Direction
predominatinge0
Value
44.65 %–62.49 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“linoleic acid predominating (44.65 %–62.49 %)”

✓ located verbatim in the source · abstract, characters 877–922 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Walnut protein content varied from 17.12 % to 20.88 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
Protein contente0
Value
17.12 % and 20.88 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Protein content varied between 17.12 % and 20.88 %”

✓ located verbatim in the source · abstract, characters 924–974 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Walnut carbohydrate content varied from 8.48 % to 24.43 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
carbohydrate contente0
Value
8.48 % and 24.43 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“carbohydrate content between 8.48 % and 24.43 %”

✓ located verbatim in the source · abstract, characters 980–1027 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Walnut ash content ranged from 1.12 % to 2.21 %.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
Ash contente0
Value
1.12 % to 2.21 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Ash content ranged from 1.12 % to 2.21 %”

✓ located verbatim in the source · abstract, characters 1029–1069 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total flavonoid content varied from 5.43 to 26.66 mg g⁻¹ QE.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
total flavonoid contente0
Value
5.43 to 26.66 mg g⁻¹ QEe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“total flavonoid content varied from 5.43 to 26.66 mg g⁻¹ QE”

✓ located verbatim in the source · abstract, characters 1077–1136 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Oil, ash, carbohydrate and flavonoid content had high heritability and expected genetic gain.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Outcome
heritability and expected genetic gaine0
Qualifier
oil, ash, carbohydrate, and flavonoid contente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“High heritability and expected genetic gain for oil, ash, carbohydrate, and flavonoid content”

✓ located verbatim in the source · abstract, characters 1138–1231 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Oil, linoleic acid and flavonoid content contributed most to variation among genotypes.

Taxon
walnut (Juglans regia L.)Juglans regiae0e1
Material
genotypese0
Outcome
total variability among genotypese0
Qualifier
oil, linoleic acid, and flavonoid contente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“oil, linoleic acid, and flavonoid content contributed most to total variability among genotypes”

✓ located verbatim in the source · abstract, characters 1337–1432 · show in text ↑
e1

“walnut (Juglans regia L.) genotypes”

✓ located verbatim in the source · abstract, characters 415–450 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors identify substantial biochemical diversity useful for developing nutritionally rich walnut cultivars.

Taxon
Walnut (Juglans regia L.)Juglans regiae0t0
Outcome
develop nutritionally rich, industrially valuable walnut cultivarse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Juglans regia
Juglans regia · TaxonBöhringer Friedrich · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“substantial biochemical diversity that can be utilized to develop nutritionally rich, industrially valuable walnut cultivars”

✓ located verbatim in the source · abstract, characters 1458–1582 · show in text ↑
t0

“Walnut (Juglans regia L.)”

✓ located verbatim in the source · title, characters 52–77 · show in text ↑