Paper · 2026 · Advances in Agriculture

Harnessing Genetic Variability for Barley ( Hordeum vulgare L.) Improvement: Insights From Yield‐Related and Phenological Trait Analysis

Seid Ebrahim Ali; Faris Hailu; Allo Aman Dido

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Open access · cc-by

Abstract

Barley is a staple food and plays a crucial role in the agricultural economy of the Ethiopian highlands. This research presents a comprehensive study on the genetic variability of barley genotypes, focusing on their yield‐related and phenological traits. During the 2022/2023 cropping season, a total of 313 barley genotypes were evaluated in four locations with an Augmented Randomized Complete Block Design (Augmented RCBD) with a single‐row 2 m 2 plot spaced 0.30 m apart over three blocks with replication of checks across all blocks. Principal component analysis (PCA) results indicated that 73.36% of the diversity came from the first four PCs. A very high to moderately high heritability in a broad sense ( H 2 b ), together with high to moderate genetic advance (GA) as percent of mean (GAM), was recorded for number of seeds per spike (NSPS) ( H 2 b = 81.02%; GAM = 51.60%), number of fertile tillers per plant (NFTPP) ( H 2 b = 69.82%; GAM = 30.55%), spike length (SL) ( H 2 b = 56.06%; GAM = 12.78), and thousand seeds weight (TSW) ( H 2 b = 66.22 %; GAM = 10.56%). The experimental materials were categorized into five clusters, where 87.22 % of the genotypes (273) fall in clusters I (163, 52.07%), II (78, 24.92%), and I (39, 12.46%). Genotypes T35 (3400), T73 (4305), T82 (4849), T123 (208857), T157 (219020), T316 (Aruso), T317 (Abdanie), T173 (219599), T176 (219772) from cluster II, and T47 (3611) of cluster V exhibited early days of heading (DH) and maturity with grain yield (GY) above the overall mean, making them suitable choice for areas facing challenges of sufficient amount of rainfall during cropping seasons. From the result of this investigation, breeder of interest should concentrate on traits that showed very high to moderately high heritability in a broad sense ( H 2 b ) together with high to moderate GAM that will be helpful to develop better genotypes for barley yield improvement.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

The first four principal components captured 73.36% of barley diversity.

Outcome
diversitye0
Value
73.36%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“73.36% of the diversity came from the first four PCs.”

✓ located verbatim in the source · abstract, characters 597–650 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Seeds per spike had broad sense heritability of 81.02% and genetic advance of 51.60%.

Outcome
number of seeds per spike (NSPS)e0
Value
81.02%; GAM = 51.60%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“number of seeds per spike (NSPS) ( H 2 b = 81.02%; GAM = 51.60%)”

✓ located verbatim in the source · abstract, characters 818–882 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fertile tillers per plant had broad sense heritability of 69.82% and genetic advance of 30.55%.

Outcome
number of fertile tillers per plant (NFTPP)e0
Value
69.82%; GAM = 30.55%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“number of fertile tillers per plant (NFTPP) ( H 2 b = 69.82%; GAM = 30.55%)”

✓ located verbatim in the source · abstract, characters 884–959 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Spike length had broad sense heritability of 56.06% and genetic advance of 12.78.

Outcome
spike length (SL)e0
Value
56.06%; GAM = 12.78e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“spike length (SL) ( H 2 b = 56.06%; GAM = 12.78)”

✓ located verbatim in the source · abstract, characters 961–1009 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Thousand seed weight had broad sense heritability of 66.22% and genetic advance of 10.56%.

Outcome
thousand seeds weight (TSW)e0
Value
66.22 %; GAM = 10.56%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“thousand seeds weight (TSW) ( H 2 b = 66.22 %; GAM = 10.56%)”

✓ located verbatim in the source · abstract, characters 1015–1075 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The barley materials were grouped into five clusters.

Outcome
five clusterse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The experimental materials were categorized into five clusters”

✓ located verbatim in the source · abstract, characters 1077–1139 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Listed barley genotypes from cluster II flowered and matured early while exceeding mean grain yield.

Outcome
early days of heading (DH) and maturity with grain yield (GY) above the overall meane0
Direction
abovee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Genotypes T35 (3400), T73 (4305), T82 (4849), T123 (208857), T157 (219020), T316 (Aruso), T317 (Abdanie), T173 (219599), T176 (219772) from cluster II, and T47 (3611) of cluster V exhibited early days of heading (DH) and maturity with grain yield (GY) above the overall mean”

✓ located verbatim in the source · abstract, characters 1249–1523 · show in text ↑
e1

“genetic variability of barley genotypes”

✓ located verbatim in the source · abstract, characters 157–196 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Breeders are advised to focus on highly heritable traits with moderate to high genetic advance.

Outcome
barley yield improvemente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“breeder of interest should concentrate on traits that showed very high to moderately high heritability in a broad sense ( H 2 b ) together with high to moderate GAM that will be helpful to develop better genotypes for barley yield improvement.”

✓ located verbatim in the source · abstract, characters 1678–1921 · show in text ↑