Abstract
Verbascum thapsus L., commonly called Mullein, is widely used in Ayurveda for the treatment of numerous ailments. The major objective of the present study is to analyse the phytochemical composition of different plant organs of V. thapsus. UPLC/MS-QToF analysis of methanol extracts from different plant organs (inflorescence, leaf, stem, and root) of V. thapsus displayed 61 active biomarkers. Among those, 48 compounds were found in the inflorescence, 40 in the leaf, 32 in the stem, and 29 in the root of V. thapsus. The distribution of these compounds was further evaluated statistically using Venn and Heat map diagrams. Moreover, a novel reverse-phase HPLC method was developed and validated as per ICH Q2(R1) guidelines to quantify verbascoside and luteolin of V. thapsus. The method was found suitable, specific, linear (R2 > 0.999), precise, accurate, and robust. The current study’s novel findings can help with the identification and quality control of verbascoside and luteolin in plant species used in Ayurvedic medicines.
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Claims 6
Claim · model-written summary
UPLC/MS-QToF analysis of Verbascum thapsus organ extracts displayed 61 active biomarkers.
- Taxon
- V. thapsus
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Verbascum thapsuse1 - Outcome
- active biomarkerse1
- Value
- 61e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“UPLC/MS-QToF analysis of methanol extracts from different plant organs (inflorescence, leaf, stem, and root) of V. thapsus displayed 61 active biomarkers”
Claim · model-written summary
Verbascum thapsus inflorescence extract contained 48 detected compounds.
- Taxon
- V. thapsusVerbascum thapsuse2
- Organ / stage
- inflorescencee2
- Outcome
- compoundse2
- Value
- 48e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Among those, 48 compounds were found in the inflorescence, 40 in the leaf, 32 in the stem, and 29 in the root of V. thapsus”
Claim · model-written summary
Verbascum thapsus leaf extract contained 40 detected compounds.
- Taxon
- V. thapsusVerbascum thapsuse2
- Organ / stage
- leafe2
- Outcome
- compoundse2
- Value
- 40e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Among those, 48 compounds were found in the inflorescence, 40 in the leaf, 32 in the stem, and 29 in the root of V. thapsus”
Claim · model-written summary
Verbascum thapsus stem extract contained 32 detected compounds.
- Taxon
- V. thapsusVerbascum thapsuse2
- Organ / stage
- steme2
- Outcome
- compoundse2
- Value
- 32e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Among those, 48 compounds were found in the inflorescence, 40 in the leaf, 32 in the stem, and 29 in the root of V. thapsus”
Claim · model-written summary
Verbascum thapsus root extract contained 29 detected compounds.
- Taxon
- V. thapsusVerbascum thapsuse2
- Organ / stage
- roote2
- Outcome
- compoundse2
- Value
- 29e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Among those, 48 compounds were found in the inflorescence, 40 in the leaf, 32 in the stem, and 29 in the root of V. thapsus”
Claim · model-written summary
The RP-HPLC method for quantifying verbascoside and luteolin in Verbascum thapsus was validated and found linear (R2 > 0.999).
- Taxon
- V. thapsusVerbascum thapsuse3
- Outcome
- validatede3
- Value
- R2 > 0.999e4
- Qualifier
- as per ICH Q2(R1) guidelinese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“a novel reverse-phase HPLC method was developed and validated as per ICH Q2(R1) guidelines to quantify verbascoside and luteolin of V. thapsus”
e4“The method was found suitable, specific, linear (R2 > 0.999), precise, accurate, and robust”