Abstract
This study systematically analyzed the BBX gene family in kiwifruit (Actinidia chinensis) to investigate its roles in abiotic stress responses. Bioinformatics analyses were performed, including gene localization, collinearity assessment, gene structure characterization, phylogenetic reconstruction, three-dimensional protein modeling, protein-protein interaction networks, and cis-acting element screening. These approaches elucidated the evolutionary relationships and structural features of the kiwifruit BBX genes. Transcriptome data revealed differential expression patterns under boron deficiency, salt stress, and cadmium stress, visualized through heatmaps. Quantitative real-time PCR validation confirmed that AcBBX4, AcBBX5, AcBBX15, AcBBX17, and AcBBX36 were significantly differentially expressed under all three stress conditions, suggesting their coordinated function in stress adaptation. Protein-protein interaction analysis identified AcBBX17 as a central hub in the regulatory network. Promoter analysis indicated that AcBBX17 contains a high proportion of methyl jasmonate responsive elements, and functional enrichment supported its involvement in the jasmonic acid signaling pathway. Based on these findings, a jasmonic acid-mediated regulatory model centered on AcBBX17 was proposed. In summary, the BBX gene family functions as key transcriptional regulators in plant development and stress response pathways, with this study providing specific insights into its role in kiwifruit abiotic stress tolerance.
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Claims 10
Claim · model-written summary
Kiwifruit BBX genes have described evolutionary relationships and structural features.
- Taxon
- kiwifruit
Actinidia deliciosae0 - Outcome
- evolutionary relationships and structural featurese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These approaches elucidated the evolutionary relationships and structural features of the kiwifruit BBX genes.”
Claim · model-written summary
Kiwifruit BBX genes showed differential expression under boron deficiency.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- boron deficiencye0
- Outcome
- differential expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome data revealed differential expression patterns under boron deficiency, salt stress, and cadmium stress”
t0“kiwifruit”
Claim · model-written summary
Kiwifruit BBX genes showed differential expression under salt stress.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- salt stresse0
- Outcome
- differential expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome data revealed differential expression patterns under boron deficiency, salt stress, and cadmium stress”
t0“kiwifruit”
Claim · model-written summary
Kiwifruit BBX genes showed differential expression under cadmium stress.
- Taxon
- kiwifruitActinidia deliciosat0
- Treatment
- cadmium stresse0
- Outcome
- differential expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome data revealed differential expression patterns under boron deficiency, salt stress, and cadmium stress”
t0“kiwifruit”
Claim · model-written summary
AcBBX4, AcBBX5 and AcBBX15 were significantly differentially expressed under all three stresses.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcBBX4, AcBBX5, AcBBX15e0
- Outcome
- significantly differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcBBX4, AcBBX5, AcBBX15, AcBBX17, and AcBBX36 were significantly differentially expressed under all three stress conditions”
t0“kiwifruit”
Claim · model-written summary
AcBBX17 and AcBBX36 were significantly differentially expressed under all three stresses.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcBBX17, and AcBBX36e0
- Outcome
- significantly differentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcBBX4, AcBBX5, AcBBX15, AcBBX17, and AcBBX36 were significantly differentially expressed under all three stress conditions”
t0“kiwifruit”
Claim · model-written summary
AcBBX17 was a central hub in the protein interaction network.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcBBX17e0
- Outcome
- central hub in the regulatory networke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein-protein interaction analysis identified AcBBX17 as a central hub in the regulatory network.”
t0“kiwifruit”
Claim · model-written summary
The AcBBX17 promoter had a high proportion of methyl jasmonate responsive elements.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcBBX17e0
- Outcome
- high proportion of methyl jasmonate responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AcBBX17 contains a high proportion of methyl jasmonate responsive elements”
t0“kiwifruit”
Claim · model-written summary
Functional enrichment supported AcBBX17 involvement in jasmonic acid signaling.
- Taxon
- kiwifruitActinidia deliciosat0
- Compound
- jasmonic acide0
- Outcome
- involvement in the jasmonic acid signaling pathwaye0
Not reportedGeneDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“functional enrichment supported its involvement in the jasmonic acid signaling pathway.”
t0“kiwifruit”
Claim · model-written summary
Authors proposed a jasmonic acid-mediated regulatory model centered on AcBBX17.
- Taxon
- kiwifruitActinidia deliciosat0
- Gene
- AcBBX17e0
- Outcome
- jasmonic acid-mediated regulatory modele0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a jasmonic acid-mediated regulatory model centered on AcBBX17 was proposed.”
t0“kiwifruit”