Abstract
Soil salinity affects the normal growth and development of crops, resulting in reduced crop yields. In the process of long-term adaptation, plants have evolved targeted salt-resistant molecular mechanisms. The calcineurin B-like protein (CBL) and CBL-interaction protein kinase (CIPK) are involved in plant response to salt stress. In this study, we identified an AtSOS2 homologous gene ZmCIPK24-2. The results of real time-qPCR showed that ZmCIPK24-2 gene was ubiquitously expressed in maize, especially in pollen, and was induced by salt stress. It was found that ZmCIPK24-2 could partially complement the salt-sensitive phenotype of the atsos2 mutant. The survival rate and the root length of ZmCIPK24-2-overexpressed lines were significantly increased compared with atsos2 mutant under high salt concentration. Subcellular localization experiments showed that ZmCIPK24-2 was localized in cytoplasm, cell membrane, and nuclear membrane. Yeast two-hybrid experiments and LUC complementation imaging assays showed that ZmCIPK24-2 was interacted with ZmCBL1, ZmCBL4, ZmCBL8, and ZmCBL9, respectively. This study provides a new experimental evidence for the functional analysis of CBL-CIPK signaling pathway in maize.
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Claims 11
Claim · model-written summary
ZmCIPK24-2 was identified as an AtSOS2 homolog in maize.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“we identified an AtSOS2 homologous gene ZmCIPK24-2”
Claim · model-written summary
ZmCIPK24-2 was expressed throughout maize, especially in pollen.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 gene was ubiquitously expressed in maize, especially in pollen”
Claim · model-written summary
Salt stress induced ZmCIPK24-2 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 gene was ubiquitously expressed in maize, especially in pollen, and was induced by salt stress”
Claim · model-written summary
ZmCIPK24-2 partially complemented the salt-sensitive atsos2 phenotype.
- Material
- atsos2 mutante0
- Treatment
- ZmCIPK24-2e0
- Gene
- ZmCIPK24-2e0
- Outcome
- salt-sensitive phenotypee0
- Direction
- partially complemente0
- Comparator
- atsos2 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 could partially complement the salt-sensitive phenotype of the atsos2 mutant”
Claim · model-written summary
ZmCIPK24-2 overexpression significantly increased survival rate versus atsos2 under high salt.
- Material
- ZmCIPK24-2-overexpressed linese0
- Treatment
- ZmCIPK24-2-overexpressed linese0
- Gene
- ZmCIPK24-2e0
- Outcome
- survival ratee0
- Direction
- increasede0
- Comparator
- atsos2 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“The survival rate and the root length of ZmCIPK24-2-overexpressed lines were significantly increased compared with atsos2 mutant under high salt concentration”
Claim · model-written summary
ZmCIPK24-2 overexpression significantly increased root length versus atsos2 under high salt.
- Material
- ZmCIPK24-2-overexpressed linese0
- Treatment
- ZmCIPK24-2-overexpressed linese0
- Gene
- ZmCIPK24-2e0
- Outcome
- root lengthe0
- Direction
- increasede0
- Comparator
- atsos2 mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“The survival rate and the root length of ZmCIPK24-2-overexpressed lines were significantly increased compared with atsos2 mutant under high salt concentration”
Claim · model-written summary
ZmCIPK24-2 localized to cytoplasm, cell membrane, and nuclear membrane.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 was localized in cytoplasm, cell membrane, and nuclear membrane”
Claim · model-written summary
ZmCIPK24-2 interacted with ZmCBL1.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 was interacted with ZmCBL1, ZmCBL4, ZmCBL8, and ZmCBL9”
Claim · model-written summary
ZmCIPK24-2 interacted with ZmCBL4.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 was interacted with ZmCBL1, ZmCBL4, ZmCBL8, and ZmCBL9”
Claim · model-written summary
ZmCIPK24-2 interacted with ZmCBL8.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 was interacted with ZmCBL1, ZmCBL4, ZmCBL8, and ZmCBL9”
Claim · model-written summary
ZmCIPK24-2 interacted with ZmCBL9.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“maize”
e0“ZmCIPK24-2 was interacted with ZmCBL1, ZmCBL4, ZmCBL8, and ZmCBL9”
The same questions in other papers
salinity · molecular: expression · Zea mays (maize)Positive and negative results
21 papers in the same direction as this paper · 10 in the opposite direction
- negative
- negative
- negative
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