Abstract
Chloroplast protein import across the outer and inner envelope membranes is mediated successively by two protein translocons termed TOC and TIC, respectively. While compositions of these megacomplexes are broadly conserved in plants, there are interesting exceptions. One such is the case of Passiflora edulis, whose previous transcriptomic studies anticipated rapid evolutionary changes in components being involved in chloroplast protein import. However, their biochemical characterizations are completely lacking. Here, we show that Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34. Interestingly, non-photosynthetic type Tic20 was identified in the envelope membranes of leaf chloroplasts, despite the absence of typical photosynthetic type TIC constituents. Hence, in Passiflora edulis, non-photosynthetic type TIC may function also in photosynthetic tissues and collaborate with the well-conserved TOC.
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Claims 5
Claim · model-written summary
Passiflora edulis chloroplasts possess an 800-1000 kDa TOC complex.
- Taxon
- Passiflora edulis
Passiflora edulise0 - Outcome
- TOCe0
- Value
- 800-1000 kDae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34”
Claim · model-written summary
The TOC complex of Passiflora edulis contains well-conserved Toc159, Toc75 and Toc34.
- Taxon
- Passiflora edulisPassiflora edulise0
- Gene
- Toc159, Toc75, and Toc34e0
- Outcome
- TOCe0
- Qualifier
- well-conservede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34”
Claim · model-written summary
Non-photosynthetic type Tic20 is identified in the envelope membranes of leaf chloroplasts of Passiflora edulis.
- Taxon
- Passiflora edulisPassiflora edulise0
- Organ / stage
- leaf chloroplastse1
- Gene
- Tic20e1
- Outcome
- envelope membranese1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34”
e1“non-photosynthetic type Tic20 was identified in the envelope membranes of leaf chloroplasts”
Claim · model-written summary
Passiflora edulis leaf chloroplasts lack typical photosynthetic type TIC constituents.
- Taxon
- Passiflora edulisPassiflora edulise0
- Outcome
- typical photosynthetic type TIC constituentse2
- Qualifier
- absencee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34”
e2“despite the absence of typical photosynthetic type TIC constituents”
Claim · model-written summary
Non-photosynthetic type TIC may function in photosynthetic tissues and collaborate with the well-conserved TOC in Passiflora edulis.
- Taxon
- Passiflora edulisPassiflora edulise0
- Organ / stage
- photosynthetic tissuese3
- Outcome
- function also in photosynthetic tissuese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Passiflora edulis chloroplasts possess 800-1000 kDa large TOC containing well-conserved Toc159, Toc75, and Toc34”
e3“non-photosynthetic type TIC may function also in photosynthetic tissues and collaborate with the well-conserved TOC”