Paper · 2026 · Journal of the Science of Food and Agriculture

Optimization of light spectrum and intensity to enhance growth and metabolite profiles in green and purple radish microgreens cultivated in a vertical farming system

Cristian Hernández‐Adasme; Vicente Martínez; Mónica Flores; et al.

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Open access · cc-by

Abstract

Abstract BACKGROUND Microgreens have emerged as a promising crop in vertical farming due to their high nutritional value and short growth cycles. Light spectrum and intensity are critical factors influencing biomass production and metabolic activity in plants, particularly in controlled environments such as vertical farming systems. This study aimed to evaluate the combined effects of light spectrum and intensity on the agronomic traits and metabolite profiles of green and purple radish ( Raphanus sativus L.) microgreens. RESULTS Three commercial LED spectra – NS12 (R:B = 1.9), Ph2.1 (R:B = 2.1) and AP673L (R:B = 5.5) – were tested at three intensities (100, 200, and 300 μmol m −2 s −1 ) in a controlled vertical farming system. Growth parameters (yield, dry matter percentage (DMP), leaf area), photosynthetic pigments, sugars, organic acids, and glucosinolates were quantified. The highest R:B ratio (5.5) under the AP673L lamp at 300 μmol m −2 s −1 significantly enhanced yield and DMP in both cultivars, while the lowest R:B ratio observed in the NS12 lamp at the same intensity maximized sugar and organic acid accumulation in green microgreens. Glucosinolate content was spectrum‐ and cultivar‐dependent: green radish accumulated higher levels under the lowest R:B ratio (NS12), whereas purple radish responded better to the R:B = 2.1 ratio under the Ph2.1 lamp at lower intensities. CONCLUSION These results demonstrate that optimal light strategies in vertical farming must be tailored to specific microgreen genotypes and light conditions to simultaneously maximize productivity and nutritional quality. © 2026 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Claims 5

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The highest R:B ratio (5.5) under the AP673L lamp at 300 μmol m −2 s −1 significantly enhanced yield and dry matter percentage in both radish cultivars.

Treatment
The highest R:B ratio (5.5) under the AP673L lamp at 300 μmol m −2 s −1e0
Outcome
yield and DMPe0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The highest R:B ratio (5.5) under the AP673L lamp at 300 μmol m −2 s −1 significantly enhanced yield and DMP in both cultivars”

✓ located verbatim in the source · abstract, characters 889–1015 · show in text ↑
t0

“radish”

✓ located verbatim in the source · title, characters 107–113 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The lowest R:B ratio under the NS12 lamp at the same intensity maximized sugar and organic acid accumulation in green radish microgreens.

Material
greene0
Treatment
lowest R:B ratio observed in the NS12 lampe0
Outcome
sugar and organic acid accumulatione0
Direction
maximizede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“the lowest R:B ratio observed in the NS12 lamp at the same intensity maximized sugar and organic acid accumulation in green microgreens”

✓ located verbatim in the source · abstract, characters 1023–1158 · show in text ↑
t0

“radish”

✓ located verbatim in the source · title, characters 107–113 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Green radish accumulated higher glucosinolate levels under the lowest R:B ratio (NS12).

Material
green radishe1
Treatment
lowest R:B ratio (NS12)e1
Outcome
Glucosinolate contente0
Direction
highere1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Glucosinolate content”

✓ located verbatim in the source · abstract, characters 1160–1181 · show in text ↑
e1

“green radish accumulated higher levels under the lowest R:B ratio (NS12)”

✓ located verbatim in the source · abstract, characters 1220–1292 · show in text ↑
t0

“radish”

✓ located verbatim in the source · title, characters 107–113 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Purple radish responded better, in glucosinolate content, to the R:B = 2.1 ratio under the Ph2.1 lamp at lower intensities.

Material
purple radishe1
Treatment
R:B = 2.1 ratio under the Ph2.1 lamp at lower intensitiese1
Outcome
Glucosinolate contente0
Direction
bettere1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Glucosinolate content”

✓ located verbatim in the source · abstract, characters 1160–1181 · show in text ↑
e1

“purple radish responded better to the R:B = 2.1 ratio under the Ph2.1 lamp at lower intensities”

✓ located verbatim in the source · abstract, characters 1302–1397 · show in text ↑
t0

“radish”

✓ located verbatim in the source · title, characters 107–113 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Optimal light strategies in vertical farming must be tailored to specific microgreen genotypes and light conditions to maximize productivity and nutritional quality.

Outcome
optimal light strategiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Raphanus sativus
Raphanus sativus · TaxonSelf, en:User:Jengod · Public domain · Wikimedia Commons ↗

Verbatim

e0

“optimal light strategies in vertical farming must be tailored to specific microgreen genotypes and light conditions”

✓ located verbatim in the source · abstract, characters 1441–1556 · show in text ↑
t0

“radish”

✓ located verbatim in the source · title, characters 107–113 · show in text ↑