Paper · 2026 · Journal of Agriculture and Food Research

Effects of CO2 enrichment and light spectrum on lettuce growth, morphology, and metabolites in controlled environment agriculture

Oluwafemi Dare Adaramola; Patrick Yawo Kpai; Philip Wiredu Addo; et al.

13
claims
25
verbatim quotes
0
citations
3
research areas
✓
Open access

Abstract

Controlled environment agriculture offers opportunities to regulate environmental factors that enhance crop productivity and quality. This study aimed to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa). Environmental parameters were controlled at 21 °C during the 18 h light period and 19 °C during the 6 h dark period, with 65% relative humidity. After 28 days, fresh mass, dry mass, plant height, head diameter, chlorophyll content, leaf area, specific leaf area, and anthocyanin content were measured. CO2 enrichment significantly (p < 0.05) increased fresh mass, dry mass, leaf area, and specific leaf area, with the largest increases observed at 800 ppm CO2. Light treatments had a significant (p < 0.05) effect on fresh mass and plant height. The combined narrow amber and blue spectrum yielded the highest fresh and dry mass, the white, far-red and UV-A combination promoted taller plants, and broad-spectrum white light produced the largest head diameters. Chlorophyll content did not show statistically significant differences across CO2 or light treatments, whereas anthocyanin content differed significantly among light treatments (p = 0.04), with the narrow amber and blue treatment producing the highest anthocyanin content. Specific leaf area decreased significantly with rising CO2 concentrations. Leaf area increased significantly at 800 ppm but plateaued at 1200 ppm (p < 0.05). These findings suggest that CO2 enrichment at 800 ppm, combined with a narrow amber and blue light spectrum, enhances indoor lettuce production by improving both biomass and quality.

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Claims 13

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

CO2 enrichment increased fresh mass and dry mass of lettuce, with the largest increases at 800 ppm.

Taxon
lettuceLactuca sativae0
Treatment
CO2 enrichmente4
Outcome
fresh mass, dry masse4
Direction
increasede4
Qualifier
largest increases observed at 800 ppm CO2e4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e4

“CO2 enrichment significantly (p < 0.05) increased fresh mass, dry mass, leaf area, and specific leaf area, with the largest increases observed at 800 ppm CO2”

✓ located verbatim in the source · abstract, characters 700–857 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

CO2 enrichment increased leaf area and specific leaf area of lettuce.

Taxon
lettuceLactuca sativae0
Treatment
CO2 enrichmente4
Outcome
leaf area, and specific leaf areae4
Direction
increasede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e4

“CO2 enrichment significantly (p < 0.05) increased fresh mass, dry mass, leaf area, and specific leaf area, with the largest increases observed at 800 ppm CO2”

✓ located verbatim in the source · abstract, characters 700–857 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Specific leaf area of lettuce decreased significantly with rising CO2 concentrations.

Taxon
lettuceLactuca sativae0
Treatment
rising CO2 concentrationse5
Outcome
Specific leaf areae5
Direction
decreasede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e5

“Specific leaf area decreased significantly with rising CO2 concentrations”

✓ located verbatim in the source · abstract, characters 1433–1506 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Light treatments significantly affected fresh mass and plant height of lettuce.

Taxon
lettuceLactuca sativae0
Treatment
Light treatmentse6
Outcome
fresh mass and plant heighte6

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e6

“Light treatments had a significant (p < 0.05) effect on fresh mass and plant height”

✓ located verbatim in the source · abstract, characters 859–942 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Leaf area increased significantly at 800 ppm CO2.

Taxon
lettuceLactuca sativae0
Treatment
800 ppme1
Outcome
Leaf areae1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“Leaf area increased significantly at 800 ppm but plateaued at 1200 ppm (p < 0.05)”

✓ located verbatim in the source · abstract, characters 1508–1589 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Leaf area plateaued at 1200 ppm CO2 relative to 800 ppm.

Taxon
lettuceLactuca sativae0
Treatment
1200 ppme1
Outcome
Leaf areae1
Direction
plateauede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“Leaf area increased significantly at 800 ppm but plateaued at 1200 ppm (p < 0.05)”

✓ located verbatim in the source · abstract, characters 1508–1589 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combined narrow amber and blue spectrum gave the highest fresh and dry mass of lettuce.

Taxon
lettuceLactuca sativae0
Treatment
combined narrow amber and blue spectrume7
Outcome
fresh and dry masse7
Direction
higheste7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e7

“The combined narrow amber and blue spectrum yielded the highest fresh and dry mass”

✓ located verbatim in the source · abstract, characters 944–1026 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The white, far-red and UV-A combination promoted taller lettuce plants.

Taxon
lettuceLactuca sativae0
Treatment
white, far-red and UV-A combinatione1
Outcome
taller plantse1
Direction
promotede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“the white, far-red and UV-A combination promoted taller plants, and broad-spectrum white light produced the largest head diameters”

✓ located verbatim in the source · abstract, characters 1028–1158 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Broad-spectrum white light gave the largest head diameters of lettuce.

Taxon
lettuceLactuca sativae0
Treatment
broad-spectrum white lighte1
Outcome
head diameterse1
Direction
largeste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“the white, far-red and UV-A combination promoted taller plants, and broad-spectrum white light produced the largest head diameters”

✓ located verbatim in the source · abstract, characters 1028–1158 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Chlorophyll content of lettuce did not differ significantly across CO2 or light treatments.

Taxon
lettuceLactuca sativae0
Treatment
CO2 or light treatmentse1
Outcome
Chlorophyll contente1
Direction
no_changeinferrede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“Chlorophyll content did not show statistically significant differences across CO2 or light treatments”

✓ located verbatim in the source · abstract, characters 1160–1261 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Anthocyanin content of lettuce differed significantly among light treatments.

Taxon
lettuceLactuca sativae0
Treatment
light treatmentse1
Outcome
anthocyanin contente1
Qualifier
p = 0.04e1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“anthocyanin content differed significantly among light treatments (p = 0.04)”

✓ located verbatim in the source · abstract, characters 1271–1347 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The narrow amber and blue light treatment gave the highest anthocyanin content in lettuce.

Taxon
lettuceLactuca sativae0
Treatment
narrow amber and blue treatmente1
Outcome
anthocyanin contente1
Direction
higheste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“to investigate the effects of CO2 enrichment (400, 800, and 1200 ppm) and light spectrum (broad-spectrum white, combined narrow amber + blue, and combined white + far-red + ultraviolet-A) on the growth and development of lettuce (Lactuca sativa)”

✓ located verbatim in the source · abstract, characters 151–396 · show in text ↑
e1

“with the narrow amber and blue treatment producing the highest anthocyanin content”

✓ located verbatim in the source · abstract, characters 1349–1431 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

CO2 enrichment at 800 ppm with a narrow amber and blue spectrum is suggested to enhance indoor lettuce production by improving biomass and quality.

Taxon
lettuceLactuca sativae1
Treatment
CO2 enrichment at 800 ppme1
Outcome
indoor lettuce productione1
Setting
indoore1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e1

“These findings suggest that CO2 enrichment at 800 ppm, combined with a narrow amber and blue light spectrum, enhances indoor lettuce production by improving both biomass and quality”

✓ located verbatim in the source · abstract, characters 1591–1772 · show in text ↑

co2 · physiology: pigments · Lactuca sativa (garden lettuce)Results differ

9 papers: 6 positive, 3 negative or no effect

2 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

co2 · growth · Lactuca sativa (garden lettuce)Positive and negative results

42 papers: 33 positive, 9 negative or no effect

32 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

38 papers more in this group

See all 42 papers →

light · physiology: pigments · Lactuca sativa (garden lettuce)Positive and negative results

52 papers: 44 positive, 8 negative or no effect

7 papers in the same direction as this paper · 44 in the opposite direction

  1. positive
  2. positive
  3. positive

48 papers more in this group

See all 52 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.