Abstract
Cerasus campanulata Maxim. possesses high ornamental value owing to its early flowering period, holding considerable application potential. However, research on its propagation techniques is still in the preliminary stage. To establish an efficient regeneration system for C. campanulata , this study used tissue-cultured seedlings as explants and employed an orthogonal experimental design to investigate the effects of key factors at different culture stages on plant regeneration. The results indicated that the suitable proliferation medium was ½MS supplemented with 0.1 mg/L NAA, 0.5 mg/L 6-BA, and 2.5 g/L banana powder. With this medium, the survival rate reached 100%, the proliferation rate was 96.67%, the proliferation coefficient exceeded 4, and the average bud height was 4.57 cm. Furthermore, the regenerated buds exhibited vigorous and healthy growth. The suitable rooting medium was ½MS, which yielded a rooting rate of over 88% and a survival rate of over 88% after greenhouse acclimatization. After 120 days of pot cultivation, the regenerated plants achieved an average plant height of 57.83 cm, an average leaf length of 15.03 cm, an average leaf width of 6.31 cm, and an average of 17 leaves per plant. Genetic stability analysis of the regenerated plantlets after three subculture cycles showed no obvious genetic variation. In conclusion, this study successfully established a practical regeneration system for C. campanulata , covering proliferation, rooting, acclimatization, pot cultivation, and preliminary genetic stability verification, which provides a reliable technical basis for the large-scale propagation and application of this species.
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Claims 12
Claim · model-written summary
The suitable proliferation medium for C. campanulata was half-strength MS with 0.1 mg/L NAA, 0.5 mg/L 6-BA and 2.5 g/L banana powder.
- Taxon
- Cerasus campanulata
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Prunus campanulatat0 - Treatment
- ½MS supplemented with 0.1 mg/L NAA, 0.5 mg/L 6-BA, and 2.5 g/L banana powdere0
- Outcome
- suitable proliferation mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the suitable proliferation medium was ½MS supplemented with 0.1 mg/L NAA, 0.5 mg/L 6-BA, and 2.5 g/L banana powder”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
On the suitable proliferation medium the survival rate reached 100%.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- survival ratee0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With this medium, the survival rate reached 100%”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
On the suitable proliferation medium the proliferation rate was 96.67%.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- proliferation ratee0
- Value
- 96.67%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proliferation rate was 96.67%”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
On the suitable proliferation medium the proliferation coefficient exceeded 4.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- proliferation coefficiente0
- Value
- exceeded 4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proliferation coefficient exceeded 4”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
On the suitable proliferation medium the average bud height was 4.57 cm.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- average bud heighte0
- Value
- 4.57 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the average bud height was 4.57 cm”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
The suitable rooting medium was half-strength MS, which gave a rooting rate of over 88%.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Treatment
- ½MSe0
- Outcome
- rooting ratee0
- Value
- over 88%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The suitable rooting medium was ½MS, which yielded a rooting rate of over 88%”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
Rooted plantlets had a survival rate of over 88% after greenhouse acclimatization.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- survival ratee0
- Value
- over 88%e0
- Setting
- greenhouse acclimatizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a survival rate of over 88% after greenhouse acclimatization”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
After 120 days of pot cultivation the regenerated plants had an average plant height of 57.83 cm.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- average plant heighte0
- Value
- 57.83 cme0
- Qualifier
- 120 days of pot cultivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 120 days of pot cultivation, the regenerated plants achieved an average plant height of 57.83 cm”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
After 120 days of pot cultivation the regenerated plants had an average leaf length of 15.03 cm.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- average leaf lengthe0
- Value
- 15.03 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an average leaf length of 15.03 cm”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
After 120 days of pot cultivation the regenerated plants had an average leaf width of 6.31 cm.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- average leaf widthe0
- Value
- 6.31 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an average leaf width of 6.31 cm”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
After 120 days of pot cultivation the regenerated plants had an average of 17 leaves per plant.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- leaves per plante0
- Value
- 17e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an average of 17 leaves per plant”
t0“Cerasus campanulata Maxim”
Claim · model-written summary
Genetic stability analysis of regenerated plantlets after three subculture cycles showed no obvious genetic variation.
- Taxon
- Cerasus campanulataPrunus campanulatat0
- Outcome
- genetic variatione0
- Qualifier
- after three subculture cyclese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic stability analysis of the regenerated plantlets after three subculture cycles showed no obvious genetic variation”
t0“Cerasus campanulata Maxim”