Abstract
Summary Stomata have the crucial role of balancing the uptake of CO 2 for photosynthesis and water loss via transpiration by adjusting their aperture. In C 3 plants, coordination between photosynthesis and stomatal conductance can be disrupted by mutations in Calvin–Benson–Bassham (CBB) cycle expression, but it is not clear whether the disruption of the C 4 carbon concentrating mechanism would have similar effects. In this study, it was hypothesized that perturbing the C 4 cycle, resulting in reduced carbon flux into the bundle sheath cells and overall net CO 2 assimilation rates, may alter stomatal regulation. To test this hypothesis, we performed gas exchange measurements on transgenic Flaveria bidentis plants carrying an antisense construct leading to reduced nicotinamide adenine dinucleotide phosphate (NADP)‐malic enzyme (NADP‐ME) activity. Stomatal conductance was unaltered in the antisense plants at ambient CO 2 levels. However, C i was significantly increased. When measurements were instead performed at common C i , stomatal conductance was significantly higher in the antisense lines. These results demonstrate that disruption of the C 4 cycle via reduced NADP‐ME activity in F. bidentis leads to altered stomatal sensitivity to C i .
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Claims 3
Claim · model-written summary
Stomatal conductance was unaltered in antisense Flaveria bidentis plants at ambient CO 2
- Taxon
- Flaveria bidentis
_close-up_(13741736383).jpg?width=60)
Flaveria bidentise1 - Treatment
- antisense constructe1
- Outcome
- Stomatal conductancee2
- Direction
- unalterede2
- Qualifier
- at ambient CO 2 levelse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_close-up_(13741736383).jpg?width=500)
Verbatim
e1“we performed gas exchange measurements on transgenic Flaveria bidentis plants carrying an antisense construct”
e2“Stomatal conductance was unaltered in the antisense plants at ambient CO 2 levels”
Claim · model-written summary
Intercellular CO2 concentration (C i) was significantly increased in antisense Flaveria bidentis plants
- Taxon
- Flaveria bidentisFlaveria bidentise1
- Treatment
- antisense constructe1
- Outcome
- C ie3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we performed gas exchange measurements on transgenic Flaveria bidentis plants carrying an antisense construct”
e3“However, C i was significantly increased”
Claim · model-written summary
At common C i, stomatal conductance was significantly higher in antisense Flaveria bidentis lines
- Taxon
- Flaveria bidentisFlaveria bidentise1
- Treatment
- antisense constructe1
- Outcome
- stomatal conductancee4
- Direction
- highere4
- Qualifier
- at common C ie4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we performed gas exchange measurements on transgenic Flaveria bidentis plants carrying an antisense construct”
e4“When measurements were instead performed at common C i , stomatal conductance was significantly higher in the antisense lines”