Abstract
Introduction The 90-kDa heat shock protein (HSP90) acts as an essential molecular chaperone, involving plantresilience against diverse abiotic and biotic stresses. Although HSP90 genes have been studied in various plant species,they have not yet been characterized in sweetpotato. Methods In this study, we performed genome-wide identification, chromosomal localization, phylogenetic analysis, collinearity analysis, and promoter analysis of HSP90 genes in I. batatas and three closely related Ipomoea species. We also investigated gene expression patterns under cold, salt, and PEG-induced drought stresses, constructed protein-protein interaction networks, and determined the subcellular localization and functional role of IbHSP90-2 via heterologous expression in yeast. Results We identified 10 HSP90 genes in I. batatas , 11 in I. trifida , and 10 each in I.triloba and I.cordatotriloba . Chromosomal localization analysis showed an uneven distribution of HSP90 genes across 15 chromosomes. Phylogenetic and collinearity analyses grouped these genes into four subfamilies,with segmental duplication being the main factor for gene family expansion. Promoter analysis revealed multiple stress-responsive cis-acting elements, indicating that IbHSP90 genes may play a role in stress regulation. Expression analysis revealed that most IbHSP90s were downregulated under cold, salt, and PEG-induced drought stresses. Additionally, we developed a protein -protein interaction network that identified connections with heat shock factors. Experiments on subcellular localization revealed that IbHSP90-2 is present in both the cytoplasm and nucleus. Finally, heterologous expression of IbHSP90-2 in yeast compromised cold resistance. Discussion This study offers a significant resource for comprehending the evolution of plant HSP90 proteins and supports the genetic enhancement of cold tolerance in sweetpotato.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Ten HSP90 genes were identified in I. batatas.
- Taxon
- I. batatas
_Flower.jpg?width=60)
Ipomoea batatase0 - Gene
- HSP90e0
- Outcome
- HSP90 genese0
- Value
- 10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“We identified 10 HSP90 genes in I. batatas , 11 in I. trifida , and 10 each in I.triloba and I.cordatotriloba .”
Claim · model-written summary
Eleven HSP90 genes were identified in I. trifida.
- Taxon
- I. trifida
Ipomoea trifidae0 - Gene
- HSP90e0
- Outcome
- HSP90 genese0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified 10 HSP90 genes in I. batatas , 11 in I. trifida , and 10 each in I.triloba and I.cordatotriloba .”
Claim · model-written summary
Ten HSP90 genes were identified in I.triloba.
- Taxon
- I.triloba
Ipomoea trilobae0 - Gene
- HSP90e0
- Outcome
- HSP90 genese0
- Value
- 10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified 10 HSP90 genes in I. batatas , 11 in I. trifida , and 10 each in I.triloba and I.cordatotriloba .”
Claim · model-written summary
Ten HSP90 genes were identified in I.cordatotriloba.
- Taxon
- I.cordatotriloba
_(6804020555).jpg?width=60)
Ipomoea cordatotrilobae0 - Gene
- HSP90e0
- Outcome
- HSP90 genese0
- Value
- 10e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(6804020555).jpg?width=500)
Verbatim
e0“We identified 10 HSP90 genes in I. batatas , 11 in I. trifida , and 10 each in I.triloba and I.cordatotriloba .”
Claim · model-written summary
HSP90 genes were unevenly distributed across 15 chromosomes.
IpomoeaOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromosomal localization analysis showed an uneven distribution of HSP90 genes across 15 chromosomes.”
t0“four Ipomoea species”
Claim · model-written summary
Phylogenetic and collinearity analyses grouped HSP90 genes into four subfamilies.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic and collinearity analyses grouped these genes into four subfamilies,with segmental duplication being the main factor for gene family expansion.”
t0“four Ipomoea species”
Claim · model-written summary
Segmental duplication was the main factor in HSP90 family expansion.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic and collinearity analyses grouped these genes into four subfamilies,with segmental duplication being the main factor for gene family expansion.”
t0“four Ipomoea species”