Abstract
Abstract Plant viruses employ diverse strategies to hijack host machinery for viral translation initiation. Cap-independent translation enhancers located in the 3′ untranslated region (3′-CITEs) have been identified in many members of the families Tombusviridae and Luteoviridae . However, viral proteins that regulate 3′-CITE-mediated translation remain unreported, and the underlying mechanisms are still poorly understood. Closteroviruses are important pathogens that infect many economically significant crops, yet their translation initiation mechanisms remain largely unexplored. Areca palm velarivirus 1 (APV1), a member of the family Closteroviridae , is the causative agent of yellow leaf disease (YLD) in areca palms. Here, we demonstrate that eukaryotic initiation factor 4E (eIF4E) specifically binds to the 3′-UTR of APV1. The APV1-encoded protein P25 interacts with eIF4E, enhances its stability, alters its subcellular localization, and promotes its recruitment to APV1 virions. Notably, P25 significantly enhances cap-independent viral translation. Knockout of eIF4E in Nicotiana benthamiana or mutation of key residues in APV1-P25 required for eIF4E interaction impairs APV1 infection. We identify the 3′-UTR of APV1 as the first reported 3′-CITE in the family Closteroviridae . Our study reveals P25 as the first viral regulator of 3′-CITE-mediated translation initiation, providing new insights into the molecular mechanisms underlying viral infection.
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Claims 9
Claim · model-written summary
specifically binds to the 3′-UTR of APV1 was reported in the APV1 translation or infection study.
- Taxon
- Areca palm
Dypsis lutescenst0 - Interactor
- APV1e0pathogen
- Gene
- eukaryotic initiation factor 4E (eIF4E)e0
- Outcome
- specifically binds to the 3′-UTR of APV1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we demonstrate that eukaryotic initiation factor 4E (eIF4E) specifically binds to the 3′-UTR of APV1.”
t0“Areca palm”
Claim · model-written summary
interacts with eIF4E was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e0pathogen
- Gene
- P25e0
- Outcome
- interacts with eIF4Ee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The APV1-encoded protein P25 interacts with eIF4E, enhances its stability, alters its subcellular localization, and promotes its recruitment to APV1 virions.”
t0“Areca palm”
Claim · model-written summary
stability was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e0pathogen
- Gene
- P25e0
- Outcome
- stabilitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The APV1-encoded protein P25 interacts with eIF4E, enhances its stability, alters its subcellular localization, and promotes its recruitment to APV1 virions.”
t0“Areca palm”
Claim · model-written summary
subcellular localization was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e0pathogen
- Gene
- P25e0
- Outcome
- subcellular localizatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The APV1-encoded protein P25 interacts with eIF4E, enhances its stability, alters its subcellular localization, and promotes its recruitment to APV1 virions.”
t0“Areca palm”
Claim · model-written summary
recruitment to APV1 virions was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e0pathogen
- Gene
- P25e0
- Outcome
- recruitment to APV1 virionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The APV1-encoded protein P25 interacts with eIF4E, enhances its stability, alters its subcellular localization, and promotes its recruitment to APV1 virions.”
t0“Areca palm”
Claim · model-written summary
cap-independent viral translation was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e1pathogen
- Gene
- P25e0
- Outcome
- cap-independent viral translatione0
- Direction
- enhancese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Notably, P25 significantly enhances cap-independent viral translation.”
t0“Areca palm”
e1“Here, we demonstrate that eukaryotic initiation factor 4E (eIF4E) specifically binds to the 3′-UTR of APV1.”
Claim · model-written summary
APV1 infection was reported in the APV1 translation or infection study.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Knockout of eIF4Ee0
- Interactor
- APV1e0pathogen
- Gene
- eIF4Ee0
- Outcome
- APV1 infectione0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of eIF4E in Nicotiana benthamiana or mutation of key residues in APV1-P25 required for eIF4E interaction impairs APV1 infection.”
Claim · model-written summary
APV1 infection was reported in the APV1 translation or infection study.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- mutation of key residues in APV1-P25e0
- Interactor
- APV1e0pathogen
- Gene
- APV1-P25e0
- Outcome
- APV1 infectione0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of eIF4E in Nicotiana benthamiana or mutation of key residues in APV1-P25 required for eIF4E interaction impairs APV1 infection.”
Claim · model-written summary
first reported 3′-CITE was reported in the APV1 translation or infection study.
- Taxon
- Areca palmDypsis lutescenst0
- Interactor
- APV1e0pathogen
- Gene
- 3′-UTR of APV1e0
- Outcome
- first reported 3′-CITEe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identify the 3′-UTR of APV1 as the first reported 3′-CITE in the family Closteroviridae .”
t0“Areca palm”
The same questions in other papers
loss of function · organism · Nicotiana benthamianaPositive and negative results
6 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.