Abstract
Branch number is an important agronomic trait that determines the number of siliques per plant and yield in rapeseed (Brassica napus). However, the complex genetic and molecular mechanisms regulating the formation of branch number in rapeseed remain unclear. In a previous study, we isolated an EMS-induced multi-branching mutant (mbm1). Here, agronomic trait analysis showed that the mbm1 mutant produced more effective primary branches, total silique number, and yield per plant compared with the wild type. Genetic analysis indicated that the multi-branching trait of the mbm1 mutant is controlled by incompletely dominant gene(s) and follows nuclear inheritance. As a result of combined BSA-seq-based mapping and RNA-seq profiling, two candidate genes, BnaC03G0462000ZS (BnaC03.GAE6) and BnaC03G0491900ZS (BnaC03.MEE14), were identified based on sequence variation and expression differences. KASP marker analysis confirmed that there are significant associations between these two gene loci and the multi-branching trait. Hybrid progeny derived from crosses between the mbm1 mutant and commercial cultivars L329 and ZS11 showed significantly improved yield due to increased effective branch number. Together, these results provide important genetic resources and a theoretical foundation for further elucidating the genetic regulatory network of branching in rapeseed and for developing high-yielding rapeseed varieties with ideal plant architecture.
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Claims 5
Claim · model-written summary
The mbm1 mutant of Brassica napus produced more effective primary branches, total silique number and yield per plant than the wild type.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Material
- mbm1 mutante0
- Outcome
- effective primary branches, total silique number, and yield per plante0
- Direction
- moree0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
t0“Brassica napus”
e0“the mbm1 mutant produced more effective primary branches, total silique number, and yield per plant compared with the wild type”
Claim · model-written summary
Genetic analysis indicated that the multi-branching trait of the mbm1 mutant is controlled by incompletely dominant gene(s) with nuclear inheritance.
- Taxon
- Brassica napusBrassica napust0
- Material
- mbm1 mutante1
- Gene
- incompletely dominant gene(s)e1
- Outcome
- multi-branching traite1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e1“the multi-branching trait of the mbm1 mutant is controlled by incompletely dominant gene(s) and follows nuclear inheritance”
Claim · model-written summary
BSA-seq mapping combined with RNA-seq identified two candidate genes, BnaC03G0462000ZS (BnaC03.GAE6) and BnaC03G0491900ZS (BnaC03.MEE14), based on sequence variation and expression differences.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnaC03G0462000ZS (BnaC03.GAE6) and BnaC03G0491900ZS (BnaC03.MEE14)e2
- Outcome
- candidate genese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e2“two candidate genes, BnaC03G0462000ZS (BnaC03.GAE6) and BnaC03G0491900ZS (BnaC03.MEE14), were identified based on sequence variation and expression differences”
Claim · model-written summary
KASP marker analysis confirmed significant associations between the two candidate gene loci and the multi-branching trait.
- Taxon
- Brassica napusBrassica napust0
- Gene
- gene locie3
- Outcome
- multi-branching traite3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e3“KASP marker analysis confirmed that there are significant associations between these two gene loci and the multi-branching trait”
Claim · model-written summary
Hybrid progeny from crosses of the mbm1 mutant with cultivars L329 and ZS11 showed significantly improved yield owing to increased effective branch number.
- Taxon
- Brassica napusBrassica napust0
- Material
- Hybrid progenye4
- Outcome
- yielde4
- Direction
- improvede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e4“Hybrid progeny derived from crosses between the mbm1 mutant and commercial cultivars L329 and ZS11 showed significantly improved yield due to increased effective branch number”