Paper · 2026 · Applied and Environmental Soil Science

The E4/E6 Absorbance Ratio, Humification Index in Soil and Cereals Drought Tolerance After 26 Years of Fertilisation

Ondřej Sedlář; Jiří Balík; Jindřich Černý; et al.

6
claims
13
verbatim quotes
0
citations
1
research areas
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Open access · cc-by

Abstract

Applying organic fertilisers improves soil health and plant growth by improving the soil organic matter, soil structure, nutrient uptake and water‐holding capacity. Small‐plot stationary field experiments on four different soils in Central Europe evaluate the long‐term effect of organic amendments and/or mineral fertilisers on the soil organic matter quality under conventional tillage and the potato/maize–winter wheat–spring barley crop rotation. Soil samples were collected after the spring barley harvest in 2022. A total nitrogen dose of 330 kg N·ha −1 over 3 years was applied in all fertilised treatments; organic fertilisers were applied after the barley harvest. The E4/E6 absorbance ratio showed significant correlations with the C‐HA/C‐FA ratio, humification index and proportion of humic acid carbon. However, when assessing the effects of fertilisation, treatments within a single homogeneous field, the E4/E6 ratio did not prove to be a reliable indicator of soil organic matter quality. Application of farmyard manure resulted in higher values of the C‐HA/C‐FA ratio, humification index and percentage of humic acid carbon compared to mineral fertilisation (N, NPK). In contrast, sewage sludge application was less effective in increasing the C‐HA/C‐FA ratio and proportion of humic acid carbon than the combination of mineral nitrogen fertilisation with straw. Regardless of weather conditions during stem elongation and flowering, higher yields of spring barley and winter wheat were recorded when nitrogen was applied in mineral fertilisers compared to manure or sewage sludge.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 6

Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The E4/E6 absorbance ratio correlated significantly with the humification index and the proportion of humic acid carbon.

Outcome
humification indexe1
Qualifier
significant correlationse2

Not reportedTaxon

Verbatim

e1

“humification index and proportion of humic acid carbon”

✓ located verbatim in the source · abstract, characters 759–813 · show in text ↑
e2

“The E4/E6 absorbance ratio showed significant correlations”

✓ located verbatim in the source · abstract, characters 674–732 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

The E4/E6 ratio was not a reliable indicator of soil organic matter quality when comparing fertilisation treatments within one homogeneous field.

Outcome
reliable indicator of soil organic matter qualitye3
Qualifier
treatments within a single homogeneous fielde5

Not reportedTaxon

Verbatim

e3

“the E4/E6 ratio did not prove to be a reliable indicator of soil organic matter quality”

✓ located verbatim in the source · abstract, characters 915–1002 · show in text ↑
e5

“treatments within a single homogeneous field”

✓ located verbatim in the source · abstract, characters 869–913 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Farmyard manure gave a higher humification index than mineral fertilisation.

Treatment
farmyard manuree1
Outcome
humification indexe3
Direction
highere1
Comparator
mineral fertilisatione2

Not reportedTaxon

Verbatim

e1

“Application of farmyard manure resulted in higher values”

✓ located verbatim in the source · abstract, characters 1004–1060 · show in text ↑
e2

“compared to mineral fertilisation (N, NPK)”

✓ located verbatim in the source · abstract, characters 1140–1182 · show in text ↑
e3

“humification index and percentage of humic acid carbon”

✓ located verbatim in the source · abstract, characters 1085–1139 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Sewage sludge application was less effective than mineral nitrogen fertilisation with straw in increasing the proportion of humic acid carbon.

Treatment
sewage sludge applicatione1
Outcome
proportion of humic acid carbone2
Direction
less effectivee1
Comparator
the combination of mineral nitrogen fertilisation with strawe2

Not reportedTaxon

Verbatim

e1

“sewage sludge application was less effective in increasing”

✓ located verbatim in the source · abstract, characters 1197–1255 · show in text ↑
e2

“proportion of humic acid carbon than the combination of mineral nitrogen fertilisation with straw”

✓ located verbatim in the source · abstract, characters 1280–1377 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Spring barley yields were higher when nitrogen was applied in mineral fertilisers than with manure or sewage sludge, regardless of weather during stem elongation and flowering.

Taxon
spring barleyHordeum vulgaree4
Treatment
nitrogen was applied in mineral fertiliserse4
Outcome
yieldse4
Direction
highere4
Qualifier
Regardless of weather conditions during stem elongation and floweringe5
Comparator
manure or sewage sludgee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“higher yields of spring barley and winter wheat were recorded when nitrogen was applied in mineral fertilisers compared to manure or sewage sludge”

✓ located verbatim in the source · abstract, characters 1450–1596 · show in text ↑
e5

“Regardless of weather conditions during stem elongation and flowering”

✓ located verbatim in the source · abstract, characters 1379–1448 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Winter wheat yields were higher when nitrogen was applied in mineral fertilisers than with manure or sewage sludge, regardless of weather during stem elongation and flowering.

Taxon
winter wheatTriticum aestivume4
Treatment
nitrogen was applied in mineral fertiliserse4
Outcome
yieldse4
Direction
highere4
Qualifier
Regardless of weather conditions during stem elongation and floweringe5
Comparator
manure or sewage sludgee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“higher yields of spring barley and winter wheat were recorded when nitrogen was applied in mineral fertilisers compared to manure or sewage sludge”

✓ located verbatim in the source · abstract, characters 1450–1596 · show in text ↑
e5

“Regardless of weather conditions during stem elongation and flowering”

✓ located verbatim in the source · abstract, characters 1379–1448 · show in text ↑

nitrogen · yield · Hordeum vulgare (barley)Positive and negative results

299 papers: 252 positive, 47 negative or no effect

251 papers in the same direction as this paper · 44 in the opposite direction · 3 reporting no difference from an alternative

  1. negative
  2. no effect
  3. negative

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fertilization · yield · Hordeum vulgare (barley)Positive and negative results

550 papers: 473 positive, 77 negative or no effect

472 papers in the same direction as this paper · 66 in the opposite direction · 11 reporting no difference from an alternative

  1. no effect
  2. negative
  3. negative

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nitrogen · yield · Triticum aestivum (wheat)Positive and negative results

2,061 papers: 1,737 positive, 324 negative or no effect

1,736 papers in the same direction as this paper · 285 in the opposite direction · 39 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

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fertilization · yield · Triticum aestivum (wheat)Positive and negative results

3,438 papers: 3,115 positive, 323 negative or no effect

3,114 papers in the same direction as this paper · 284 in the opposite direction · 39 reporting no difference from an alternative

  1. positive
  2. no effect
  3. negative

3,434 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.