Abstract
The search for new antibacterial agents is often hindered by the limited efficiency of traditional isolation-based workflows, which may result in low yields or insufficient detection of bioactive constituents. To address these limitations, an affordable two-dimensional thin-layer chromatography (2D-TLC) combined with direct bioautography was applied for the rapid screening of the hydroalcoholic leaf extract of Myrtus communis. The 2D-TLC-UV/Vis/FLD-Bacillus subtilis assay provided improved separation capacity, including in overloaded conditions, also enabling the visualization of minor antibacterial zones. Bioactivity-guided HRMS/MS analysis performed directly from the inhibition areas allowed the tentative identification of several triterpenoids and fifteen myrtucommulone-type non-prenylated acylphloroglucinol derivatives. Some of these metabolites have not been previously described in the context of antibacterial screening of M. communis. This is the first application of an eight-dimensional workflow integrating 2D-TLC-UV/Vis/FLD-B. subtilis bioassay-HESI-HRMS/MS for the screening and characterization of antibacterials in a complex matrix, exemplified here for M. communis, which provided previously unexplored compounds with potent antibacterial activity.
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Claims 3
Claim · model-written summary
The 2D-TLC bioautography workflow tentatively identified fifteen myrtucommulone-type acylphloroglucinol derivatives in Myrtus communis leaf extract.
- Taxon
- Myrtus communis
_in_Athens_on_July_4%2C_2023.jpg?width=60)
Myrtus communise0 - Outcome
- tentative identificatione1
- Value
- fifteene1
- Qualifier
- myrtucommulone-type non-prenylated acylphloroglucinol derivativese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_in_Athens_on_July_4%2C_2023.jpg?width=500)
Verbatim
e0“hydroalcoholic leaf extract of Myrtus communis”
e1“tentative identification of several triterpenoids and fifteen myrtucommulone-type non-prenylated acylphloroglucinol derivatives”
Claim · model-written summary
The workflow tentatively identified several triterpenoids in Myrtus communis leaf extract.
- Taxon
- Myrtus communisMyrtus communise0
- Outcome
- tentative identificatione1
- Qualifier
- several triterpenoidse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydroalcoholic leaf extract of Myrtus communis”
e1“tentative identification of several triterpenoids and fifteen myrtucommulone-type non-prenylated acylphloroglucinol derivatives”
Claim · model-written summary
Identified compounds showed potent antibacterial activity.
- Taxon
- Myrtus communisMyrtus communise0
- Outcome
- antibacterial activitye3
- Qualifier
- potente3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hydroalcoholic leaf extract of Myrtus communis”
e3“previously unexplored compounds with potent antibacterial activity”