Abstract
Two hundred and twenty trees (150 apples and 70 hazelnuts) from seven locations in Serbia were tested for the presence of Ilarvirus ApMV (apple mosaic virus). ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves. ELISA-positive samples were further tested by RT-PCR with two different primer pairs. One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut. Sequence analysis of amplified PCR fragments of the selected isolates confirmed that Serbian ApMV isolates from apple share the highest nucleotide identity (98.66 ‒ 99.02%) with isolates from India and Canada.
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Claims 5
Claim · model-written summary
ELISA confirmed apple mosaic virus (ApMV) in 11 apple trees (7.3%) in Serbia, with typical and severe mosaic symptoms on the leaves.
- Taxon
- apples
Malus domesticae0 - Interactor
- ApMV
Ilarvirus ApMVe0pathogen - Outcome
- presence of ApMVe0
- Value
- 7.3%e0
- Qualifier
- typical and severe mosaic symptoms on the leavese0
- Setting
- Serbiae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves”
e1“seven locations in Serbia”
Claim · model-written summary
ELISA confirmed apple mosaic virus (ApMV) in six hazelnut trees (8.5%) in Serbia, with typical and severe mosaic symptoms on the leaves.
- Interactor
- ApMVIlarvirus ApMVe0pathogen
- Outcome
- presence of ApMVe0
- Value
- 8.5%e0
- Qualifier
- typical and severe mosaic symptoms on the leavese0
- Setting
- Serbiae1
CorylusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves”
e1“seven locations in Serbia”
Claim · model-written summary
One RT-PCR primer pair was efficient for detecting ApMV isolates from apple.
- Taxon
- appleMalus domesticae0
- Interactor
- ApMVIlarvirus ApMVe0pathogen
- Outcome
- RT-PCR detection of ApMV isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut”
Claim · model-written summary
A different RT-PCR primer pair was efficient for detecting ApMV isolates from hazelnut.
- Taxon
- hazelnute0
- Interactor
- ApMVIlarvirus ApMVe0pathogen
- Outcome
- RT-PCR detection of ApMV isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut”
Claim · model-written summary
Serbian ApMV isolates from apple shared the highest nucleotide identity (98.66-99.02%) with isolates from India and Canada.
- Taxon
- appleMalus domesticae0
- Interactor
- ApMVIlarvirus ApMVe0pathogen
- Outcome
- nucleotide identitye0
- Value
- 98.66 ‒ 99.02%e0
- Qualifier
- isolates from India and Canadae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Serbian ApMV isolates from apple share the highest nucleotide identity (98.66 ‒ 99.02%) with isolates from India and Canada”