Paper · Institutional Repositories DataBase (IRDB)

Introduction of WAG, a Wheat AGAMOUS Homolog, Reduces Corolla Size in Torenia

間, 竜太郎; 村井, 耕二; 岸本, 早苗; et al.

4
claims
8
verbatim quotes
0
citations
2
research areas
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Open access

Abstract

The function of a 1.1-kb transcript of WAG, a wheat AGAMOUS homolog, has not been clarified.To analyze its function, it was fused with the cauliflower mosaic virus 35S promoter and introduced into torenia (Torenia fournieri).Torenia transformants grew normally with regular plant height, size and shape of leaves, and development of inflorescence.However, the corolla was significantly smaller in 10 transformants out of 21.Generally, the vertical and horizontal diameters of the lip part of the corolla, as well as the length of the corolla (including the tube part), were all reduced in the smaller flowers, suggesting that the size of the corolla was reduced equally in all dimensions.Southern blot analysis showed the existence of the WAG gene in the genomes of these plants.We report that WAG reduces the size of the corolla in torenia.

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Claims 4

Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Torenia transformants had normal plant height, leaves, and inflorescence development.

Taxon
ToreniaToreniae0
Material
transformantse0
Outcome
regular plant height, size and shape of leaves, and development of inflorescencee0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Torenia
Torenia · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Torenia transformants grew normally with regular plant height, size and shape of leaves, and development of inflorescence”

✓ located verbatim in the source · abstract, characters 225–346 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Ten of 21 transformants had significantly smaller corollas.

Taxon
toreniaToreniae1
Treatment
Introduction of WAGt0
Gene
WAGt0
Outcome
corollae0
Direction
smallere0
Value
10 transformants out of 21e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Torenia
Torenia · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the corolla was significantly smaller in 10 transformants out of 21”

✓ located verbatim in the source · abstract, characters 356–423 · show in text ↑
e1

“introduced into torenia (Torenia fournieri)”

✓ located verbatim in the source · abstract, characters 181–224 · show in text ↑
t0

“Introduction of WAG”

✓ located verbatim in the source · title, characters 0–19 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Vertical and horizontal lip diameters and total corolla length were reduced in the smaller flowers.

Taxon
toreniaToreniae1
Organ / stage
corollae0
Outcome
vertical and horizontal diameters of the lip part of the corolla, as well as the length of the corollae0
Direction
reducede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Torenia
Torenia · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the vertical and horizontal diameters of the lip part of the corolla, as well as the length of the corolla (including the tube part), were all reduced in the smaller flowers”

✓ located verbatim in the source · abstract, characters 435–608 · show in text ↑
e1

“introduced into torenia (Torenia fournieri)”

✓ located verbatim in the source · abstract, characters 181–224 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Southern blotting detected WAG in the transformant genomes.

Taxon
toreniaToreniae1
Gene
WAGe0
Outcome
existence of the WAG gene in the genomes of these plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Torenia
Torenia · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Southern blot analysis showed the existence of the WAG gene in the genomes of these plants”

✓ located verbatim in the source · abstract, characters 688–778 · show in text ↑
e1

“introduced into torenia (Torenia fournieri)”

✓ located verbatim in the source · abstract, characters 181–224 · show in text ↑