Abstract
Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum). Recent studies indicated that the Va gene (Ntab0942120) determines the susceptibility of the crop to PVY, and that the Va gene product interacts with the PVY genome-linked protein (VPg) to initiate the PVY genome translation process, which ultimately leads to the systemic infection of tobacco by the virus. In this research, tobacco cultivar LJ911 was used as receptor material for gene editing. Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established. Pathology tests indicated that the edited plants had gained PVY resistance. Therefore, the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY- resistant tobacco.
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Claims 3
Claim · model-written summary
CRISPR/Cas9 knockout of Va yielded transgene-free homozygous edited T1 tobacco plants.
- Taxon
- tobacco
Nicotiana tabacumt0 - Material
- LJ911t0
- Organ / stage
- T1 generatione0
- Treatment
- CRISPR/Cas9 technologye0
- Gene
- Vae0
- Outcome
- transgene-free homozygous edited plantse0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene Va was knocked out through CRISPR/Cas9 technology, and transgene-free homozygous edited plants in the T1 generation were established.”
t0“tobacco LJ911”
Claim · model-written summary
Edited tobacco plants gained PVY resistance in pathology tests.
- Taxon
- tobaccoNicotiana tabacumt0
- Material
- LJ911t0
- Treatment
- CRISPR/Cas9 technologye1
- Interactor
- Potato virus Y
Potato virus Ye2pathogen - Gene
- Vae1
- Outcome
- PVY resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pathology tests indicated that the edited plants had gained PVY resistance.”
e1“Gene Va was knocked out through CRISPR/Cas9 technology”
t0“tobacco LJ911”
e2“Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum).”
Claim · model-written summary
The edited tobacco material is potentially useful for breeding PVY-resistant tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- Potato virus YPotato virus Ye1pathogen
- Outcome
- potentially useful genetic resources for breeding of PVY- resistant tobaccoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the edited materials generated in this study represent potentially useful genetic resources for breeding of PVY- resistant tobacco.”
e1“Potato virus Y (PVY) is one of the main diseases of tobacco (Nicotiana tabacum).”
The same questions in other papers
loss of function · Potato virus Y · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.