Abstract
Eukaryotic translation-initiation factors eIF4E and eIF(iso)4E in plants play key roles in infection by potyviruses and other plant RNA viruses. Mutations in the genes encoding these factors reduce susceptibility to the viruses, and are the basis of several recessive virus resistance genes widely used in plant breeding. Because virus variants occasionally break such resistance, the molecular basis for this process must be elucidated. Although deletion mutants of eIF4E1-S of tobacco (Nicotiana tabacum L.) resist Potato virus Y (PVY; the type member of the genus Potyvirus), resistance-breaking strains of PVY threaten tobacco production worldwide. Here, we used RNA interference technology to knock down tobacco eIF4E2-S and eIF4E2-T genes or eIF(iso)4E-S and eIF(iso)4E-T genes. Transgenic plants with reduced transcript levels of both eIF(iso)4E-S and eIF(iso)4E-T showed reduced susceptibility to a resistance-breaking PVY strain with a K105E mutation in the viral genome-associated protein (VPg). By screening a population of chemically induced mutants of eIF(iso)4E-S and eIF(iso)4E-T, we showed that plants with a nonsense mutation in eIF(iso)4E-T, but not eIF(iso)4E-S, showed reduced susceptibility to the resistance-breaking PVY strain. In a yeast two-hybrid assay, VPg of the resistance-breaking strain, but not wild-type PVY, physically interacted with the eIF(iso)4E-T protein. Thus, eIF4E1-S is required for infection by PVY, but eIF(iso)4E-T is required for infection by the resistance-breaking strain. Our study provides the first evidence for the involvement of a host eukaryotic translation-initiation factor in the infection cycle of a resistance-breaking virus strain. The eIF(iso)4E-T mutants will be useful in tobacco breeding to introduce resistance against resistance-breaking PVY strains.
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Claims 8
Claim · model-written summary
Reduced eIF(iso)4E-S and eIF(iso)4E-T transcript levels reduced tobacco susceptibility to resistance-breaking PVY.
- Taxon
- tobacco
Nicotiana tabacume1 - Treatment
- reduced transcript levels of both eIF(iso)4E-S and eIF(iso)4E-Te0
- Interactor
- PVY
Potato virus Ye0pathogen - Gene
- eIF(iso)4E-S and eIF(iso)4E-Te0
- Outcome
- susceptibility to a resistance-breaking PVY straine0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic plants with reduced transcript levels of both eIF(iso)4E-S and eIF(iso)4E-T showed reduced susceptibility to a resistance-breaking PVY strain with a K105E mutation”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
Claim · model-written summary
A nonsense mutation in eIF(iso)4E-T reduced susceptibility to resistance-breaking PVY.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- nonsense mutation in eIF(iso)4E-Te0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- eIF(iso)4E-Te0
- Outcome
- susceptibility to the resistance-breaking PVY straine0
- Direction
- reducede0
- Comparator
- eIF(iso)4E-Se0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants with a nonsense mutation in eIF(iso)4E-T, but not eIF(iso)4E-S, showed reduced susceptibility to the resistance-breaking PVY strain”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
Claim · model-written summary
An eIF(iso)4E-S mutation did not reduce susceptibility to resistance-breaking PVY.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- eIF(iso)4E-Se0
- Interactor
- PVYPotato virus Ye2pathogen
- Outcome
- susceptibility to the resistance-breaking PVY straine0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants with a nonsense mutation in eIF(iso)4E-T, but not eIF(iso)4E-S, showed reduced susceptibility to the resistance-breaking PVY strain”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
e2“resistance-breaking PVY strain with a K105E mutation”
Claim · model-written summary
VPg from resistance-breaking PVY physically interacted with tobacco eIF(iso)4E-T in a yeast two-hybrid assay.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- eIF(iso)4E-Te0
- Outcome
- physically interacted with the eIF(iso)4E-T proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VPg of the resistance-breaking strain, but not wild-type PVY, physically interacted with the eIF(iso)4E-T protein”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
Claim · model-written summary
Wild-type PVY VPg did not physically interact with tobacco eIF(iso)4E-T.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- wild-type PVYe0pathogen
- Gene
- eIF(iso)4E-Te0
- Outcome
- not wild-type PVY, physically interacted with the eIF(iso)4E-T proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VPg of the resistance-breaking strain, but not wild-type PVY, physically interacted with the eIF(iso)4E-T protein”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
Claim · model-written summary
eIF4E1-S is required for PVY infection.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- eIF4E1-Se0
- Outcome
- infection by PVYe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eIF4E1-S is required for infection by PVY”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
Claim · model-written summary
eIF(iso)4E-T is required for resistance-breaking PVY infection.
- Taxon
- tobaccoNicotiana tabacume1
- Interactor
- PVYPotato virus Ye2pathogen
- Gene
- eIF(iso)4E-Te0
- Outcome
- infection by the resistance-breaking straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eIF(iso)4E-T is required for infection by the resistance-breaking strain”
e1“tobacco (Nicotiana tabacum L.) resist Potato virus Y”
e2“resistance-breaking PVY strain with a K105E mutation”
Claim · model-written summary
The authors propose eIF(iso)4E-T mutants for tobacco breeding against resistance-breaking PVY.
- Taxon
- tobaccoNicotiana tabacume0
- Interactor
- PVYPotato virus Ye0pathogen
- Gene
- eIF(iso)4E-Te0
- Outcome
- tobacco breeding to introduce resistance against resistance-breaking PVY strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The eIF(iso)4E-T mutants will be useful in tobacco breeding to introduce resistance against resistance-breaking PVY strains”
The same questions in other papers
loss of function · Potato virus Y · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.