Abstract
Serine protease-like proteases (SBTs) constitute a distinct class of serine proteases exclusive to plants. Despite the recognized importance of SBTs in various plants, knowledge concerning the evolution and function of SBT genes in Brassica napus is limited. In this study, a total of 140, 63, and 71 SBT genes were identified in B. napus, B. oleracea, and B. rapa, respectively. Phylogenetic analysis classified these 330 identified SBTs into five subfamilies, and collinearity analyses further indicated that gene redundancy and gene loss were strongly associated with polyploidization in Brassicaceae plants. Additionally, analyses of gene structure and conserved motifs suggested that evolutionary changes in exon-intron structures may contribute to the differentiation of coding regions, expression patterns, and even functions within the BnSBT family. Analysis of promoter cis-regulatory elements revealed their predominant association with hormonal responses, abiotic stress, and processes related to plant growth and development. Furthermore, eight differentially expressed genes (DEGs) were identified through a comparative analysis of RNA-Seq data from high- and low-yielding cultivars. qRT-PCR verification also revealed that these eight DEGs (BnSBT1.4b, BnSBT1.4c, BnSBT1.4d, BnSBT1.5c, BnSBT1.6b, BnSBT1.8a, BnSBT3.14a, and BnSBT3.14b) were significantly differentially expressed in the pericarp and seeds. They could be categorized into two distinct groups: BnSBT1.4b, BnSBT1.4c, BnSBT1.4d, BnSBT1.5c, and BnSBT1.8a were highly expressed in high-SPSI material, whereas BnSBT1.6b, BnSBT3.14a, and BnSBT3.14b were highly expressed in low-SPSI material. These results suggest that BnSBTs have diverse potential functions in regulating yield traits in Brassica napus. These findings offer key insights into Brassicaceae SBT genes and highlight the importance of BnSBTs in achieving high yields in Brassica napus.
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Claims 12
Claim · model-written summary
140 SBT genes were identified in B. napus.
- Taxon
- B. napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Gene
- SBT genese0
- Outcome
- identifiede0
- Value
- 140e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“a total of 140, 63, and 71 SBT genes were identified in B. napus, B. oleracea, and B. rapa, respectively”
Claim · model-written summary
63 SBT genes were identified in B. oleracea.
- Taxon
- B. oleracea
Brassica oleraceae0 - Gene
- SBT genese0
- Outcome
- identifiede0
- Value
- 63e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 140, 63, and 71 SBT genes were identified in B. napus, B. oleracea, and B. rapa, respectively”
Claim · model-written summary
71 SBT genes were identified in B. rapa.
- Taxon
- B. rapa
Brassica rapae0 - Gene
- SBT genese0
- Outcome
- identifiede0
- Value
- 71e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 140, 63, and 71 SBT genes were identified in B. napus, B. oleracea, and B. rapa, respectively”
Claim · model-written summary
Phylogenetic analysis classified the 330 identified SBTs into five subfamilies.
Not reportedTaxon
Verbatim
e0“Phylogenetic analysis classified these 330 identified SBTs into five subfamilies”
Claim · model-written summary
Collinearity analyses indicated that gene redundancy and gene loss were strongly associated with polyploidization in Brassicaceae plants.
- Taxon
- Brassicaceae plantsBrassicaceaee0
- Treatment
- polyploidizatione0
- Outcome
- gene redundancy and gene losse0
Not reportedDirection
Verbatim
e0“collinearity analyses further indicated that gene redundancy and gene loss were strongly associated with polyploidization in Brassicaceae plants”
Claim · model-written summary
Analyses of gene structure and conserved motifs suggested that evolutionary changes in exon-intron structures may contribute to differentiation of coding regions, expression patterns and functions within the BnSBT family.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnSBT familye0
- Outcome
- differentiation of coding regions, expression patterns, and even functionse0
- Qualifier
- evolutionary changes in exon-intron structurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“analyses of gene structure and conserved motifs suggested that evolutionary changes in exon-intron structures may contribute to the differentiation of coding regions, expression patterns, and even functions within the BnSBT family”
Claim · model-written summary
Promoter cis-regulatory elements were predominantly associated with hormonal responses, abiotic stress and processes related to plant growth and development.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- promoter cis-regulatory elementse0
- Qualifier
- hormonal responses, abiotic stress, and processes related to plant growth and developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“Analysis of promoter cis-regulatory elements revealed their predominant association with hormonal responses, abiotic stress, and processes related to plant growth and development”
Claim · model-written summary
Eight differentially expressed genes were identified by comparing RNA-Seq data of high- and low-yielding cultivars.
- Taxon
- Brassica napusBrassica napust0
- Material
- high- and low-yielding cultivarse0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“eight differentially expressed genes (DEGs) were identified through a comparative analysis of RNA-Seq data from high- and low-yielding cultivars”
Claim · model-written summary
qRT-PCR verified that the eight DEGs were significantly differentially expressed in the pericarp and seeds.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- pericarp and seedse1
- Gene
- eight DEGse0
- Outcome
- differentially expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“qRT-PCR verification also revealed that these eight DEGs”
e1“were significantly differentially expressed in the pericarp and seeds”
Claim · model-written summary
BnSBT1.4b, BnSBT1.4c, BnSBT1.4d, BnSBT1.5c and BnSBT1.8a were highly expressed in high-SPSI material.
- Taxon
- Brassica napusBrassica napust0
- Material
- high-SPSI materiale0
- Gene
- BnSBT1.4b, BnSBT1.4c, BnSBT1.4d, BnSBT1.5c, and BnSBT1.8ae0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“BnSBT1.4b, BnSBT1.4c, BnSBT1.4d, BnSBT1.5c, and BnSBT1.8a were highly expressed in high-SPSI material”
Claim · model-written summary
BnSBT1.6b, BnSBT3.14a and BnSBT3.14b were highly expressed in low-SPSI material.
- Taxon
- Brassica napusBrassica napust0
- Material
- low-SPSI materiale0
- Gene
- BnSBT1.6b, BnSBT3.14a, and BnSBT3.14be0
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassica napus”
e0“BnSBT1.6b, BnSBT3.14a, and BnSBT3.14b were highly expressed in low-SPSI material”
Claim · model-written summary
BnSBTs have diverse potential functions in regulating yield traits in Brassica napus.
- Taxon
- Brassica napusBrassica napuse0
- Gene
- BnSBTse0
- Outcome
- yield traitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnSBTs have diverse potential functions in regulating yield traits in Brassica napus”