Paper · 2025 · Dialnet (Universidad de la Rioja)

Micropropagation of Billbergia pallidiflora, a bromeliad with ornamental potential

Marín López, Lizbeth; Valdez Hernández, Edna; Hernández Castro, Carlos Gilberto

5
claims
11
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Billbergia pallidiflora Liemb. belongs to the Bromeliaceae family. In Mexico, it is distributed in several states and extends to El Salvador and Nicaragua. The main objective of this project was to standardize multiplication for the species through in vitro culture. The goal is to generate a protocol for its legal cultivation, given its morphological characteristics that make it ideal for ornamental use. The aim is to offer a sustainable alternative that reduces pressure on wild populations, a common problem in many bromeliads To this end, the effect of naphthaleneacetic acid (NAA) and benzyladenine (BA) on its micropropagation were evaluated from microstem fragments cultivated for a period of 60 days. The study was established in a completely randomized design with five treatments and three replicates, each experimental unit consisted of 10 flasks with two explants per flask. The data obtained were analyzed using the Tukey means test with a confidence level of α=0.05, in the SAS statistical package. Each microstem fragment generated at least one whole plant. 50 % of the explants regenerated more than one shoot. Statistically significant differences were found between treatments for shoot length, aerial dry matter, root length, and root volume variables; for the effect of NAA and BA there was no statistical effect. B. pallidiflora proved to be a viable plant for micropropagation over a 60-day period, using microstem fragments, even without addition of plant growth regulators.

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Each microstem fragment generated at least one whole plant.

Taxon
Billbergia pallidifloraBillbergia pallidiflorat0
Organ / stage
microstem fragmente0
Outcome
whole plante0
Value
at least onee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Billbergia pallidiflora
Billbergia pallidiflora · TaxonFrancisco Emilio Roldán Velasco · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Each microstem fragment generated at least one whole plant.”

✓ located verbatim in the source · abstract, characters 1016–1075 · show in text ↑
t0

“Billbergia pallidiflora”

✓ located verbatim in the source · title, characters 20–43 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Half of the explants regenerated more than one shoot.

Taxon
Billbergia pallidifloraBillbergia pallidiflorat0
Organ / stage
explantse0
Outcome
regenerated more than one shoote0
Value
50 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Billbergia pallidiflora
Billbergia pallidiflora · TaxonFrancisco Emilio Roldán Velasco · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“50 % of the explants regenerated more than one shoot.”

✓ located verbatim in the source · abstract, characters 1076–1129 · show in text ↑
t0

“Billbergia pallidiflora”

✓ located verbatim in the source · title, characters 20–43 · show in text ↑
Intervention effectobservedoriginal resultmixedsignificant (as stated)measured

Claim · model-written summary

Treatments differed significantly in shoot length, aerial dry matter, root length and root volume.

Taxon
Billbergia pallidifloraBillbergia pallidiflorat0
Treatment
treatmentse0
Outcome
shoot length, aerial dry matter, root length, and root volumee0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Billbergia pallidiflora
Billbergia pallidiflora · TaxonFrancisco Emilio Roldán Velasco · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Statistically significant differences were found between treatments for shoot length, aerial dry matter, root length, and root volume variables”

✓ located verbatim in the source · abstract, characters 1130–1273 · show in text ↑
t0

“Billbergia pallidiflora”

✓ located verbatim in the source · title, characters 20–43 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

NAA and BA had no statistical effect on the measured propagation variables.

Taxon
Billbergia pallidifloraBillbergia pallidiflorat0
Treatment
NAA and BAe0
Outcome
micropropagatione1
Direction
no statistical effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Billbergia pallidiflora
Billbergia pallidiflora · TaxonFrancisco Emilio Roldán Velasco · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“for the effect of NAA and BA there was no statistical effect.”

✓ located verbatim in the source · abstract, characters 1275–1336 · show in text ↑
e1

“naphthaleneacetic acid (NAA) and benzyladenine (BA) on its micropropagation”

✓ located verbatim in the source · abstract, characters 560–635 · show in text ↑
t0

“Billbergia pallidiflora”

✓ located verbatim in the source · title, characters 20–43 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors conclude micropropagation is viable over 60 days with microstem fragments even without growth regulators.

Taxon
Billbergia pallidifloraBillbergia pallidiflorat0
Outcome
micropropagation over a 60-day period, using microstem fragments, even without addition of plant growth regulatorse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Billbergia pallidiflora
Billbergia pallidiflora · TaxonFrancisco Emilio Roldán Velasco · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“B. pallidiflora proved to be a viable plant for micropropagation over a 60-day period, using microstem fragments, even without addition of plant growth regulators.”

✓ located verbatim in the source · abstract, characters 1337–1500 · show in text ↑
t0

“Billbergia pallidiflora”

✓ located verbatim in the source · title, characters 20–43 · show in text ↑