Abstract
Tobacco ( Nicotiana tabacum L., cultivar MSK326) seeds after long term storage exhibit reduced seed vigor, leading to impaired seedling establishment and economic losses in tobacco production. Seed priming with nanoparticles serves as a low-cost and environmentally friendly strategy to improve seed performance. In this study, we found that silicon dioxide nanoparticles (SiO 2 NPs) priming can significantly enhance seed germination and subsequent seedling establishment of severely aged tobacco seeds. Transcriptomic analysis revealed that SiO 2 NPs priming altered the expression of genes involved in the reactive oxygen species (ROS) metabolism and phytohormone biosynthesis of severely aged tobacco seeds. Consistent with these transcriptional changes, SiO 2 NPs priming resulted in reduced superoxide anion (O 2 •- ) accumulation, increased hydrogen peroxide (H 2 O 2 ) levels, and enhanced activities of antioxidant related enzymes in severely aged tobacco seeds. Furthermore, endogenous gibberellin (GA) and auxin (IAA) contents were significantly increased following SiO 2 NPs priming in severely aged tobacco seeds. Interestingly, exogenous GA 3 and IAA can promote the seed vigor of severely aged tobacco seeds. The metabolomic analysis further corroborated the results of physiological and transcriptomic analyses that SiO 2 NPs priming restores the vigor of severely aged tobacco seeds by coordinating redox homeostasis and GA/IAA biosynthesis. Collectively, our results demonstrated the underlying mechanism by which SiO 2 NPs priming revitalizes the seed vigor of severely aged tobacco seeds and also contributes to the understanding of the physiological and molecular mechanism of SiO 2 NPs priming in plants. • Tobacco seeds after long term storage exhibit reduced seed vigor, leading to impaired seedling establishment and economic losses in tobacco production. In our study, we demonstrated that SiO 2 NPs priming can significantly enhance seed germination and subsequent seedling establishment of severely aged tobacco seeds. Further physiological and molecular mechanism revealed that SiO 2 NPs priming restores the seed vigor of severely aged tobacco seeds by coordinating redox homeostasis and GA/IAA biosynthesis. Our study may highlight the potential application of SiO 2 NPs in improving the seed vigor and seedling establishment of naturally aged tobacco seeds.
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Claims 8
Claim · model-written summary
Silicon dioxide nanoparticle priming significantly enhanced germination and seedling establishment.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- silicon dioxide nanoparticles (SiO 2 NPs) priminge0
- Outcome
- seed germination and subsequent seedling establishmente0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silicon dioxide nanoparticles (SiO 2 NPs) priming can significantly enhance seed germination and subsequent seedling establishment of severely aged tobacco seeds”
Claim · model-written summary
Priming altered expression of ROS metabolism and phytohormone biosynthesis genes.
- Outcome
- expression of genes involved in the reactive oxygen species (ROS) metabolism and phytohormone biosynthesise0
Not reportedTaxonDirection
Verbatim
e0“SiO 2 NPs priming altered the expression of genes involved in the reactive oxygen species (ROS) metabolism and phytohormone biosynthesis”
Claim · model-written summary
Priming reduced superoxide anion accumulation.
Not reportedTaxonComparator
Verbatim
e0“SiO 2 NPs priming resulted in reduced superoxide anion (O 2 •- ) accumulation”
Claim · model-written summary
Priming increased hydrogen peroxide levels.
Not reportedTaxonTreatmentComparator
Verbatim
e0“increased hydrogen peroxide (H 2 O 2 ) levels”
Claim · model-written summary
Priming enhanced antioxidant enzyme activity.
Not reportedTaxonTreatmentComparator
Verbatim
e0“enhanced activities of antioxidant related enzymes”
Claim · model-written summary
Priming significantly increased endogenous gibberellin and auxin content.
- Treatment
- SiO 2 NPs priminge0
- Outcome
- endogenous gibberellin (GA) and auxin (IAA) contentse0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“endogenous gibberellin (GA) and auxin (IAA) contents were significantly increased following SiO 2 NPs priming”
Claim · model-written summary
Exogenous GA3 promoted vigor of aged tobacco seeds.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- exogenous GA 3e0
- Outcome
- seed vigore0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA 3 and IAA can promote the seed vigor of severely aged tobacco seeds”
Claim · model-written summary
Exogenous IAA promoted vigor of aged tobacco seeds.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- IAAe0
- Outcome
- seed vigore0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA 3 and IAA can promote the seed vigor of severely aged tobacco seeds”
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8 of 9 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.