Abstract
Introduction Emilia sonchifolia L. ( E. sonchifolia ) is a traditionally medicinal plant widespread in tropical and subtropical regions and is valued for its diverse bioactive properties, including anti-inflammatory, antioxidant, and anti-tumor activities. In October 2021, two groups of E. sonchifolia —one non-symptomatic (Mock) and the other exhibiting yellow vein symptoms (Vln)—were identified from an experimental nursery in Yulin City, Guangxi Zhuang Autonomous Region, China. The virus infecting symptomatic plants was isolated, sequenced, and confirmed to be closely related to Emilia yellow vein virus Fz1 (EYVV-Fz1) from Fujian province, and was designated Emilia yellow vein virus Gx (EYVV-Gx). Methods To reveal gene expression changes and enriched biological pathways in response to begomovirus infection in the medicinal plant E. sonchifolia , transcriptome analysis between Mock and Vln plants was conducted using integrated Single Molecule Real Time (SMRT)-seq and Illumina-seq technologies. Results A total of 195 differentially expressed genes (DEGs) were detected, and enriched in pathways related to reactive oxygen species (ROS) scavenging, cell wall remodeling, hormone signaling, transcriptional regulation, as well as stress and immune related responses. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) validations revealed that gene expression was group-dependent and tissue-specific. Discussion These findings provide new insights into infection-associated transcriptional responses of E. sonchifolia under natural begomovirus infection and represent the first transcriptomic analysis of this species based on the combined use of Illumina and SMRT sequencing. This study provides a valuable resource for future molecular studies and may support improvements in cultivation strategies for medicinal plants.
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Claims 4
Claim · model-written summary
The virus from symptomatic Emilia sonchifolia plants was confirmed to be closely related to Emilia yellow vein virus Fz1 from Fujian province and designated EYVV-Gx.
- Taxon
- Emilia sonchifolia
Emilia sonchifoliat0 - Interactor
- Emilia yellow vein virus Fz1e0pathogen
- Outcome
- closely related to Emilia yellow vein virus Fz1 (EYVV-Fz1)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“confirmed to be closely related to Emilia yellow vein virus Fz1 (EYVV-Fz1) from Fujian province”
t0“Emilia sonchifolia”
Claim · model-written summary
A total of 195 differentially expressed genes were detected between mock and symptomatic Emilia sonchifolia plants.
- Taxon
- Emilia sonchifoliaEmilia sonchifoliat0
- Interactor
- begomovirus
Begomovirust1pathogen - Outcome
- differentially expressed genes (DEGs)e0
- Value
- 195e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 195 differentially expressed genes (DEGs) were detected”
t0“Emilia sonchifolia”
t1“begomovirus infection”
Claim · model-written summary
The differentially expressed genes were enriched in pathways related to ROS scavenging, cell wall remodeling, hormone signaling, transcriptional regulation, and stress and immune related responses.
- Taxon
- Emilia sonchifoliaEmilia sonchifoliat0
- Interactor
- begomovirusBegomovirust1pathogen
- Outcome
- pathways related to reactive oxygen species (ROS) scavenging, cell wall remodeling, hormone signaling, transcriptional regulation, as well as stress and immune related responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enriched in pathways related to reactive oxygen species (ROS) scavenging, cell wall remodeling, hormone signaling, transcriptional regulation, as well as stress and immune related responses”
t0“Emilia sonchifolia”
t1“begomovirus infection”
Claim · model-written summary
RT-qPCR validation showed that gene expression was group-dependent and tissue-specific.
- Taxon
- Emilia sonchifoliaEmilia sonchifoliat0
- Outcome
- gene expressione0
- Qualifier
- group-dependent and tissue-specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene expression was group-dependent and tissue-specific”
t0“Emilia sonchifolia”