Abstract
Tomato leaf mold disease caused by infection by Cladosporium fulvum is an important threat that restricts tomato production. The resistance of tomatoes to this pathogen usually depends on the Cf gene family. However, the emergence of new physiological races has resulted in resistance being overcome in many commercial cultivars that carry Cf genes, making effective resistance genes increasingly limited. While the Cf-13 gene is known to be involved in resistance to tomato leaf mold, it has not yet been cloned. In this study, genetic analysis of Ont7813 (which carries the Cf-13 gene) and Moneymaker suggested that the genetic pattern of Cf-13 is single-gene dominant inheritance. Using bulked segregant analysis sequencing (BSA-seq) and genetic mapping, we successfully mapped the tomato leaf mold resistance gene Cf-13 to a 2.4 Mb interval on chromosome 6. This target region contains 40 candidate genes harboring non-synonymous SNPs/Indels within their coding sequences. Upon analysis, we speculate that Solyc06g033920 (annotated as a receptor-like protein, RLP) is the primary candidate gene for Cf-13 . To verify its function, we specifically silenced this gene in the disease-resistant material Ont7813 using virus-induced gene silencing (VIGS) technology. The results showed that silencing Solyc06g033920 reduced the resistance of the plants to C. fulvum . This research provides important genetic resources and a theoretical basis for the exploration of disease resistance genes and the breeding of disease-resistant varieties.
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Claims 5
Claim · model-written summary
Cf-13 resistance in Ont7813 followed single-gene dominant inheritance.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- Ont7813e0
- Gene
- Cf-13e0
- Outcome
- single-gene dominant inheritancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic analysis of Ont7813 (which carries the Cf-13 gene) and Moneymaker suggested that the genetic pattern of Cf-13 is single-gene dominant inheritance”
t0“tomato”
Claim · model-written summary
Cf-13 was mapped to a 2.4 Mb interval on chromosome 6.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- Cf-13e0
- Outcome
- 2.4 Mb interval on chromosome 6e0
- Value
- 2.4 Mbe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mapped the tomato leaf mold resistance gene Cf-13 to a 2.4 Mb interval on chromosome 6”
Claim · model-written summary
The target interval contained 40 candidate genes with nonsynonymous variants.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- candidate genes harboring non-synonymous SNPs/Indelse0
- Value
- 40e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This target region contains 40 candidate genes harboring non-synonymous SNPs/Indels within their coding sequences”
t0“tomato”
Claim · model-written summary
Solyc06g033920 was proposed as the primary candidate for Cf-13.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- Solyc06g033920e0
- Outcome
- primary candidate gene for Cf-13e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we speculate that Solyc06g033920 (annotated as a receptor-like protein, RLP) is the primary candidate gene for Cf-13”
t0“tomato”
Claim · model-written summary
Silencing Solyc06g033920 reduced tomato resistance to C. fulvum.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- silencing Solyc06g033920e0
- Interactor
- C. fulvum
Fulvia fulvae0pathogen - Gene
- Solyc06g033920e0
- Outcome
- resistance of the plants to C. fulvume0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing Solyc06g033920 reduced the resistance of the plants to C. fulvum”
t0“tomato”
The same questions in other papers
loss of function · Fulvia fulva · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.