Abstract
ABSTRACT Meloidogyne enterolobii is an extremely pathogenic soil-borne pest, RNA interference (RNAi) represents a highly targeted eco-friendly control strategy, but its application is limited by poor dsRNA stability and low cellular uptake efficiency. Herein, we developed a star polycation (SPc)-based nanocarrier system to enhance the stability and cellular uptake of dsRNA for efficient control of M . enterolobii . We identified Mekk-3 as a potential candidate gene in M. enterolobii . SPc spontaneously assembled with ds Mekk-3 to form nanoscale complex that protected dsRNA from degradation by RNase A, soil extract, and nematode lysate. Compared with naked dsRNA, the ds Mekk-3 /SPc complex increased dsRNA uptake by 42.3% within 24 h, enhanced gene silencing by 1.9-fold, and significantly increased larval mortality. Transcriptome profiling further supported the involvement of endocytosis and transport-related pathways in SPc-mediated delivery, whereas Mekk-3 silencing may lead to the suppression of MAPK-related genes. In greenhouse trials, the complex reduced larval infection and gall formation. Collectively, these results identify Mekk-3 as a potential RNAi target gene, and establish SPc as a viable delivery platform for next-generation RNA-based bionematicides.
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Claims 8
Claim · model-written summary
Mekk-3 was identified as a potential nematode RNAi target.
- Taxon
- M. enterolobii
_5384315.jpg?width=60)
Meloidogyne enterolobiie0 - Gene
- Mekk-3e0
- Outcome
- potential candidate genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_5384315.jpg?width=500)
Verbatim
e0“We identified Mekk-3 as a potential candidate gene in M. enterolobii .”
Claim · model-written summary
The SPc complex protected dsRNA against degradation.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Outcome
- protected dsRNA from degradatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SPc spontaneously assembled with ds Mekk-3 to form nanoscale complex that protected dsRNA from degradation by RNase A, soil extract, and nematode lysate.”
t0“Meloidogyne enterolobii”
Claim · model-written summary
The complex increased dsRNA uptake by 42.3% within 24 h compared with naked dsRNA.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Treatment
- ds Mekk-3 /SPc complexe0
- Outcome
- dsRNA uptakee0
- Direction
- increasede0
- Value
- 42.3%e0
- Qualifier
- within 24 he0
- Comparator
- naked dsRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with naked dsRNA, the ds Mekk-3 /SPc complex increased dsRNA uptake by 42.3% within 24 h, enhanced gene silencing by 1.9-fold, and significantly increased larval mortality.”
t0“Meloidogyne enterolobii”
Claim · model-written summary
The complex enhanced gene silencing by 1.9-fold.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Treatment
- ds Mekk-3 /SPc complexe0
- Outcome
- gene silencinge0
- Direction
- enhancede0
- Value
- 1.9-folde0
- Comparator
- naked dsRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with naked dsRNA, the ds Mekk-3 /SPc complex increased dsRNA uptake by 42.3% within 24 h, enhanced gene silencing by 1.9-fold, and significantly increased larval mortality.”
t0“Meloidogyne enterolobii”
Claim · model-written summary
The complex significantly increased larval mortality.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Treatment
- ds Mekk-3 /SPc complexe0
- Outcome
- larval mortalitye0
- Direction
- increasede0
- Comparator
- naked dsRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with naked dsRNA, the ds Mekk-3 /SPc complex increased dsRNA uptake by 42.3% within 24 h, enhanced gene silencing by 1.9-fold, and significantly increased larval mortality.”
t0“Meloidogyne enterolobii”
Claim · model-written summary
Transcriptome profiling supported endocytosis and transport pathways in delivery.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Outcome
- SPc-mediated deliverye0
- Qualifier
- endocytosis and transport-related pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome profiling further supported the involvement of endocytosis and transport-related pathways in SPc-mediated delivery”
t0“Meloidogyne enterolobii”
Claim · model-written summary
Mekk-3 silencing may suppress MAPK-related genes.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Gene
- Mekk-3e0
- Outcome
- suppression of MAPK-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mekk-3 silencing may lead to the suppression of MAPK-related genes.”
t0“Meloidogyne enterolobii”
Claim · model-written summary
The complex reduced larval infection and gall formation in greenhouse trials.
- Taxon
- Meloidogyne enterolobiiMeloidogyne enterolobiit0
- Treatment
- the complexe0
- Outcome
- larval infection and gall formatione0
- Direction
- reducede0
- Setting
- greenhousee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In greenhouse trials, the complex reduced larval infection and gall formation.”
t0“Meloidogyne enterolobii”