Paper · 2026 · Nordic Journal of Botany

Leaf micromorphology and genetic diversity analysis of Iranian Hordeum species using ISSR and SCoT markers

Maryam Keshavarzi; Raheleh Tabaripour; Nasrin Farasat

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claims
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verbatim quotes
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citations
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research areas
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Open access · cc-by

Abstract

The genus Hordeum (Poaceae), of the tribe Triticeae, comprises approximately 32 species with substantial potential for barley improvement. Hordeum vulgare is an economically important cereal widely cultivated across diverse environments, from the Arctic to desert and humid regions. In this study, we analyzed 67 individuals from 11 populations representing five Iranian Hordeum species using inter‐simple sequence repeats (ISSR) and start codon targeted (SCoT) molecular markers. We evaluated the discriminatory power of these markers and identified diagnostic micromorphological traits of the abaxial leaf blade. Genetic variation and affinities among Iranian Hordeum species were assessed using molecular markers combined with micromorphology. Neighbor‐net and UPGMA analyses revealed close evolutionary relationships among Hordeum marinum , Hordeum bulbosum , and Hordeum murinum . Micromorphological examination of the abaxial leaf surface showed significant interspecific variation in both qualitative and quantitative traits. Additionally, H. bulbosum exhibited close affinities with Hordeum vulgare subsp. spontaneum and, to a lesser extent, with Hordeum brevisubulatum . While most Hordeum research has focused on biological traits, salinity tolerance, and disease resistance, breeding efforts are limited by small grain size and scarce genetic information on wild taxa. Our findings provide valuable insights for the collection, conservation, and genetic management of Hordeum germplasm, contributing to future breeding and improvement programs. Overall, this study highlights the utility of integrating molecular and micromorphological data to guide the conservation and targeted improvement of barley and related species.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Hordeum marinum, Hordeum bulbosum and Hordeum murinum have close evolutionary relationships.

Taxon
Hordeum marinumHordeum marinume0
Outcome
close evolutionary relationshipse0
Qualifier
Hordeum bulbosum , and Hordeum murinume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum marinum
Hordeum marinum · TaxonJozefsu · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Neighbor‐net and UPGMA analyses revealed close evolutionary relationships among Hordeum marinum , Hordeum bulbosum , and Hordeum murinum”

✓ located verbatim in the source · abstract, characters 747–883 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Abaxial leaf surface traits varied significantly between species.

Taxon
HordeumHordeumt0
Organ / stage
abaxial leaf surfacee0
Outcome
interspecific variation in both qualitative and quantitative traitse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum
Hordeum · TaxonMatt Lavin · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Micromorphological examination of the abaxial leaf surface showed significant interspecific variation in both qualitative and quantitative traits”

✓ located verbatim in the source · abstract, characters 886–1031 · show in text ↑
t0

“Iranian Hordeum species”

✓ located verbatim in the source · title, characters 55–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

H. bulbosum had close affinities with Hordeum vulgare subsp. spontaneum and weaker affinities with Hordeum brevisubulatum.

Taxon
H. bulbosumHordeum bulbosume0
Outcome
close affinitiese0
Qualifier
with Hordeum vulgare subsp. spontaneum and, to a lesser extent, with Hordeum brevisubulatume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum bulbosum
Hordeum bulbosum · TaxonArgo Navis · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“H. bulbosum exhibited close affinities with Hordeum vulgare subsp. spontaneum and, to a lesser extent, with Hordeum brevisubulatum”

✓ located verbatim in the source · abstract, characters 1047–1177 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Integrating molecular and micromorphological data is useful for conservation and targeted improvement of barley relatives.

Outcome
integrating molecular and micromorphological datae0
Qualifier
to guide the conservation and targeted improvemente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“this study highlights the utility of integrating molecular and micromorphological data to guide the conservation and targeted improvement of barley and related species”

✓ located verbatim in the source · abstract, characters 1565–1732 · show in text ↑