Claim · model-written summary
Across both marker systems, 160 loci were scored.
Verbatim
e0“50 Hordeum genotypes”
e1“Across both systems, 160 loci were scored, of which 138 were polymorphic”
Title
Using SCoT and SRAP Markers to Assess Genetic Diversity in Wild and Cultivated Hordeum Genotypes
Elena-Laura Contescu; Alina Gabriela Turcu; Florin Gabriel Anton Romanian Agricultural Research 2026. doi:10.59665/rar4326
Other claims from this paper (15)
- Observation Across both marker systems, 138 loci were polymorphic.
- Observation SCoT generated 79 loci.
- Observation SCoT produced 64 polymorphic loci.
- Observation SRAP generated 81 loci.
- Observation SRAP produced 77 polymorphic loci.
- Observation SCoT polymorphism per primer ranged from 75% to 100%.
- Observation SCoT PIC values were between 0.12 and 0.26.
- Observation SRAP polymorphism per combination ranged from 85.7% to 100%.
- Observation SRAP PIC values were between 0.22 and 0.33.
- Observation UPGMA clustering of the combined matrix separated cultivated controls from wild accessions.
- Observation UPGMA also identified a distinct Hb4X subgroup within H. bulbosum.
- Observation PCA supported the same genetic structure as the clustering.
- Method / resource The high cophenetic correlation confirmed strong agreement between the dendrogram and the original dissimilarity matrix.
- Method / resource Amplification profiles were clear and reproducible.
- Method / resource The combined SCoT and SRAP approach provided a robust and practical framework for germplasm structuring and parent selection.
