Abstract
To elucidate the role of β-caryophyllene emitted from rice plants in mediating interactions among rice, herbivores and their natural enemies, a rice β-caryophyllene synthase gene OsCAS was cloned, expressed in E. coli and over-expressed or silenced in rice plants using an Agrobacterium-based transformation system. The gene OsCAS, a 1731 bp cDNA, contains an open reading frame of 1728 bp, which encodes a protein of 576 amino acids with a calculated molecular weight of 66.5 kDa. Phylogenetic tree analysis showed that the putative rice OsCAS had a high amino acid identity (99%) to maize (Zea mays) β-caryophyllene synthase, whereas a low amino acid identity (51%) to those in other plants including Arabodopsis (Arabidopsis thaliana), Artemisia (Artemisia annua) and cucumber (Cucumis sativus). Infestation by rice brown planthopper Nilaparvata lugens (Stål) or jasmonic acid treatment enhanced significantly the expression levels of OsCAS gene. The recombinant OsCAS protein was expressed in E. coli and rice lines with over-expression or RNAi silencing of OsCAS gene were obtained by A grobacterium-mediated transformation, which laid a foundation for elucidating the biochemical and ecological functions of OsCAS.
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Claims 8
Claim · model-written summary
Rice OsCAS cDNA was 1731 bp with a 1728 bp open reading frame.
- Taxon
- rice plants
Oryza sativae1 - Gene
- OsCASe0
- Outcome
- cDNAe0
- Value
- 1731 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene OsCAS, a 1731 bp cDNA, contains an open reading frame of 1728 bp”
e1“silenced in rice plants using an Agrobacterium-based transformation system.”
Claim · model-written summary
OsCAS encoded 576 amino acids with calculated molecular weight 66.5 kDa.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encodes a protein of 576 amino acids with a calculated molecular weight of 66.5 kDa”
e1“The gene OsCAS”
e2“silenced in rice plants using an Agrobacterium-based transformation system.”
Claim · model-written summary
Rice OsCAS had 99% amino acid identity to maize β-caryophyllene synthase.
- Taxon
- riceOryza sativae0
- Gene
- OsCASe0
- Outcome
- amino acid identitye0
- Direction
- highe0
- Value
- 99%e0
- Comparator
- maize (Zea mays) β-caryophyllene synthasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice OsCAS had a high amino acid identity (99%) to maize (Zea mays) β-caryophyllene synthase”
Claim · model-written summary
Rice OsCAS had 51% amino acid identity to synthases in other plants.
- Taxon
- riceOryza sativae1
- Gene
- OsCASe1
- Outcome
- amino acid identitye0
- Direction
- lowe0
- Value
- 51%e0
- Comparator
- other plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low amino acid identity (51%) to those in other plants including Arabodopsis (Arabidopsis thaliana), Artemisia (Artemisia annua) and cucumber (Cucumis sativus)”
e1“rice OsCAS”
Claim · model-written summary
Brown planthopper infestation significantly enhanced OsCAS expression.
- Taxon
- riceOryza sativae0
- Treatment
- Infestation by rice brown planthopper Nilaparvata lugens (Stål)e0
- Interactor
- Nilaparvata lugens (Stål)
Nilaparvata lugense0pest - Gene
- OsCASe0
- Outcome
- expression levels of OsCAS genee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infestation by rice brown planthopper Nilaparvata lugens (Stål) or jasmonic acid treatment enhanced significantly the expression levels of OsCAS gene.”
Claim · model-written summary
Jasmonic acid significantly enhanced OsCAS expression.
- Taxon
- rice plantsOryza sativae1
- Treatment
- jasmonic acid treatmente0
- Gene
- OsCASe0
- Outcome
- expression levels of OsCAS genee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid treatment enhanced significantly the expression levels of OsCAS gene.”
e1“silenced in rice plants using an Agrobacterium-based transformation system.”
Claim · model-written summary
Recombinant OsCAS protein was expressed in E. coli.
- Taxon
- rice plantsOryza sativae1
- Gene
- OsCASe0
- Outcome
- recombinant OsCAS protein was expressed in E. colie0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant OsCAS protein was expressed in E. coli”
e1“silenced in rice plants using an Agrobacterium-based transformation system.”
Claim · model-written summary
Rice lines overexpressing or silencing OsCAS were obtained.
- Taxon
- rice plantsOryza sativae1
- Gene
- OsCASe0
- Outcome
- rice lines with over-expression or RNAi silencing of OsCAS genee0
- Value
- rice linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice lines with over-expression or RNAi silencing of OsCAS gene were obtained by A grobacterium-mediated transformation”
e1“silenced in rice plants using an Agrobacterium-based transformation system.”
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See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.