Abstract
Plants undergo several but very precise molecular, physiological, and biochemical modulations in response to biotic stresses. Mitogen-activated protein kinase (MAPK) cascades orchestrate multiple cellular processes including plant growth and development as well as plant responses against abiotic and biotic stresses. However, the role of MAPK kinases (MAPKKs/MKKs/MEKs) in the regulation of plant resistance to herbivores has not been extensively investigated. Here, we cloned a rice MKK gene, OsMKK3, and investigated its function. It was observed that mechanical wounding, infestation of brown planthopper (BPH) Nilaparvata lugens, and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3. The over-expression of OsMKK3 (oe-MKK3) increased levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA), and decreased SA levels in rice after BPH attack. Additionally, the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3. Besides, oe-MKK3 also augmented chlorophyll content but impaired plant growth. We confirm that MKK3 plays a pivotal role in the signaling pathway. It is proposed that OsMKK3 mediated positive regulation of rice resistance to BPH by means of herbivory-induced phytohormone dynamics.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
Mechanical wounding induced OsMKK3 expression in rice.
- Taxon
- Rice
Oryza sativat0 - Treatment
- mechanical woundinge0
- Gene
- OsMKK3e0
- Outcome
- expression of OsMKK3e0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mechanical wounding, infestation of brown planthopper (BPH) Nilaparvata lugens, and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3”
t0“Rice”
Claim · model-written summary
Brown planthopper infestation induced OsMKK3 expression in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- infestation of brown planthopper (BPH) Nilaparvata lugense0
- Interactor
- Nilaparvata lugens
Nilaparvata lugense0herbivore - Gene
- OsMKK3e0
- Outcome
- expression of OsMKK3e0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infestation of brown planthopper (BPH) Nilaparvata lugens, and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3”
t0“Rice”
Claim · model-written summary
Methyl jasmonate treatment induced OsMKK3 expression in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- methyl jasmonate (MeJA)e0
- Gene
- OsMKK3e0
- Outcome
- expression of OsMKK3e0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3”
t0“Rice”
Claim · model-written summary
Salicylic acid treatment induced OsMKK3 expression in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- salicylic acid (SA)e0
- Gene
- OsMKK3e0
- Outcome
- expression of OsMKK3e0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with methyl jasmonate (MeJA) or salicylic acid (SA) could induce the expression of OsMKK3”
t0“Rice”
Claim · model-written summary
OsMKK3 overexpression increased jasmonic acid, jasmonoyl-L-isoleucine and abscisic acid levels in rice after planthopper attack.
- Taxon
- riceOryza sativae0
- Treatment
- over-expression of OsMKK3 (oe-MKK3)e0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of OsMKK3 (oe-MKK3) increased levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA), and decreased SA levels in rice after BPH attack.”
Claim · model-written summary
OsMKK3 overexpression decreased salicylic acid levels in rice after planthopper attack.
- Taxon
- riceOryza sativae0
- Treatment
- over-expression of OsMKK3 (oe-MKK3)e0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- SA levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of OsMKK3 (oe-MKK3) increased levels of jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-Ile), and abscisic acid (ABA), and decreased SA levels in rice after BPH attack.”
Claim · model-written summary
OsMKK3 overexpression significantly reduced brown planthopper feeding and oviposition preference.
- Taxon
- RiceOryza sativat0
- Treatment
- over-expression of OsMKK3e0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- preference for feeding and ovipositione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3”
t0“Rice”
Claim · model-written summary
OsMKK3 overexpression significantly reduced brown planthopper egg hatching rate.
- Taxon
- RiceOryza sativat0
- Treatment
- over-expression of OsMKK3e0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- hatching rate of BPH eggse0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3”
t0“Rice”
Claim · model-written summary
OsMKK3 overexpression significantly reduced brown planthopper nymph survival rate.
- Taxon
- RiceOryza sativat0
- Treatment
- over-expression of OsMKK3e0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- BPH nymph survival ratee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the preference for feeding and oviposition, the hatching rate of BPH eggs, and BPH nymph survival rate were significantly compromised due to over-expression of OsMKK3”
t0“Rice”
Claim · model-written summary
OsMKK3 overexpression increased rice chlorophyll content.
- Taxon
- RiceOryza sativat0
- Treatment
- oe-MKK3e0
- Outcome
- chlorophyll contente0
- Direction
- augmentede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oe-MKK3 also augmented chlorophyll content but impaired plant growth”
t0“Rice”
Claim · model-written summary
OsMKK3 overexpression impaired rice growth.
- Taxon
- RiceOryza sativat0
- Treatment
- oe-MKK3e0
- Outcome
- plant growthe0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oe-MKK3 also augmented chlorophyll content but impaired plant growth”
t0“Rice”
Claim · model-written summary
The authors proposed that OsMKK3 promotes rice resistance to brown planthopper through herbivory-induced phytohormone dynamics.
- Taxon
- riceOryza sativae0
- Interactor
- BPHe0herbivore
- Gene
- OsMKK3e0
- Outcome
- rice resistance to BPHe0
- Qualifier
- herbivory-induced phytohormone dynamicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is proposed that OsMKK3 mediated positive regulation of rice resistance to BPH by means of herbivory-induced phytohormone dynamics.”
The same questions in other papers
Nilaparvata lugens · molecular: expression · Oryza sativa (rice)Positive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- negative
- positive
- positive
5 papers more in this group
See all 9 papers →infection · molecular: expression · Oryza sativa (rice)Positive and negative results
40 papers in the same direction as this paper · 16 in the opposite direction
- negative
- positive
- positive
53 papers more in this group
See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.