Abstract
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) has been detected in several types of plant cells, but the gene has not been reported in sugar cane. Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the alpha and beta subunits of this enzyme. The deduced amino acid sequences showed 76 and 80% similarity with the corresponding alpha and beta subunits of C. paradisi. A high degree of similarity was also observed among the PFK b subunits when the alignment of the sugar cane sequences was compared to those of Ricinus communis and Solanum tuberosum. It appears that alpha and beta are two distinct subunits; they were found at different concentrations in several sugar cane tissues. It remains to be determined if the different gene expression levels have some physiological importance and how they affect sucrose synthesis, export, and storage in vacuoles. A comparison between the amino acid sequences of b PFKs from a variety of organisms allowed us to identify the two critical Asp residues typical of this enzyme's activity site and the other binding sites; these residues are tightly conserved in all members of this protein family. Apparently, there are catalytic residues on the b subunit of the pyrophosphate-dependent enzyme.
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Claims 7
Claim · model-written summary
Both the alpha and beta subunits of PPi-PFK were identified in the sugar cane EST database using Citrus paradisi sequences.
Verbatim
e0“Using Citrus paradisi PPi-PFK gene (AF095520 and AF095521) sequences to search the sugar cane EST database, we have identified both the alpha and beta subunits of this enzyme”
Claim · model-written summary
The deduced amino acid sequence of the sugar cane alpha subunit showed 76% similarity to the C. paradisi alpha subunit.
Verbatim
e0“The deduced amino acid sequences showed 76 and 80% similarity with the corresponding alpha and beta subunits of C. paradisi”
t0“sugar cane”
Claim · model-written summary
The deduced amino acid sequence of the sugar cane beta subunit showed 80% similarity to the C. paradisi beta subunit.
Verbatim
e0“The deduced amino acid sequences showed 76 and 80% similarity with the corresponding alpha and beta subunits of C. paradisi”
t0“sugar cane”
Claim · model-written summary
The alpha and beta subunits are distinct subunits found at different concentrations in several sugar cane tissues.
Verbatim
e0“they were found at different concentrations in several sugar cane tissues”
Claim · model-written summary
Comparison of b PFK amino acid sequences from a variety of organisms identified two critical Asp residues typical of the enzyme activity site and other binding sites.
Verbatim
e0“A comparison between the amino acid sequences of b PFKs from a variety of organisms allowed us to identify the two critical Asp residues typical of this enzyme's activity site and the other binding sites; these residues are tightly conserved in all members of this protein family”
Claim · model-written summary
These residues are tightly conserved in all members of the protein family.
Verbatim
e0“A comparison between the amino acid sequences of b PFKs from a variety of organisms allowed us to identify the two critical Asp residues typical of this enzyme's activity site and the other binding sites; these residues are tightly conserved in all members of this protein family”
Claim · model-written summary
There appear to be catalytic residues on the b subunit of the pyrophosphate-dependent enzyme.
- Outcome
- catalytic residues on the b subunite0
Not reportedTaxon
Verbatim
e0“Apparently, there are catalytic residues on the b subunit of the pyrophosphate-dependent enzyme”