Claim · model-written summary
NaCl-tolerant calli showed decreased relative growth rate and water content compared with the control line, with higher reductions in the 150 mM tolerant callus.
Solanum tuberosumVerbatim
t0“Solanum tuberosum”
e1“The NaCl-tolerant calli showed a decrease in relative growth rate and water content, with higher reductions in the 150 mM tolerant callus”
e2“compared to control line”
Title
In vitro selection of salt tolerant cell lines in Solanum tuberosum L.
F. Queirós; F. Fidalgo; I. Santos; et al. Biologia Plantarum 2007. doi:10.1007/s10535-007-0149-y
Other claims from this paper (10)
- Observation Potato callus cell lines able to grow on media with 50, 100, 150 or 200 mM NaCl were established by direct recurrent or gradual selection.
- Observation In potato callus under direct selection only small clusters of cells survived on medium with 150 or 200 mM NaCl.
- Observation On 100 mM NaCl under direct selection, small cell portions of potato callus appeared necrotic.
- Observation Salt-tolerant potato calli obtained by gradual selection were more compact and developed a greenish colour free from necrotic areas.
- Intervention effect Lipid peroxidation was increased in 50 mM and 100 mM NaCl-tolerant calli.
- Intervention effect Lipid peroxidation in the 150 mM tolerant callus remained similar to the 100 mM values.
- Intervention effect Ascorbic acid content increased significantly in 100 mM and 150 mM NaCl-tolerant calli compared with the 50 mM line, reaching two-fold the control value.
- Observation The contents of soluble and insoluble proteins increased in the salt-tolerant potato lines.
- Observation SDS-PAGE of soluble proteins showed synthesis of specific polypeptides in the presence of NaCl in the culture medium.
- Observation SDS-PAGE showed synthesis of a new polypeptide in the salt-tolerant calli.