Claim · model-written summary
Somatic embryos from cryopreserved cell lines were matured and germinated.
- Taxon
- Douglas-fir
Pseudotsuga menziesiit0 - Organ / stage
- somatic embryose7
- Outcome
- were matured and germinatede7
Verbatim
t0“Simple and efficient protocols for the initiation and proliferation of embryogenic tissue of Douglas-fir”
e7“A sample of proliferating cell lines was successfully cryopreserved, thawed and somatic embryos were matured and germinated from these lines”
Title
Cathie Reeves; Cathy Hargreaves; Jean-François Trontin; et al. Trees 2017. doi:10.1007/s00468-017-1622-7
Other claims from this paper (9)
- Method / resource A simple and efficient protocol for initiating Douglas-fir embryogenic tissue was developed from modified previously published techniques.
- Intervention effect Disinfection of whole cones was sufficient to prevent contamination, instead of disinfecting seeds.
- Observation At optimal zygotic embryo development stages, the average initiation percentage of embryogenic tissue was 69.3% with the most effective protocol.
- Observation Proliferating Douglas-fir cell lines were challenging to maintain, with a high percentage composed of a mixture of embryonal masses and callus.
- Intervention effect 2,4-D at a lower concentration reduced the number of mixed cell lines.
- Intervention effect Repeated liquid suspension and subculture increased the number of embryogenic cell lines with sustained proliferation.
- Intervention effect Maltose in place of sucrose in the proliferation medium improved fresh mass gain.
- Intervention effect Maltose in place of sucrose in the proliferation medium consistently increased early somatic embryo patterning and growth.
- Observation Proliferating cell lines were successfully cryopreserved and thawed.