Claim · model-written summary
Q-Sepharose separation showed that the decrease in aldolase activity was due to inhibition of plastid aldolase.
- Taxon
- potato
Solanum tuberosume0 - Gene
- plastid aldolasee2
- Outcome
- decreasee2
- Qualifier
- due to inhibition of plastid aldolasee2
Verbatim
e0“Antisense expression of a full length cDNA encoding plastid aldolase led to decreased expression of aldolase at the transcript and protein level in several 'antisense' potato transformants”
e2“Separation on a Q-Sepharose-FF column showed the decrease was due to inhibition of plastid aldolase”
Title
Volker Haake; Rita Zrenner; Uwe Sonnewald; et al. The Plant Journal 1998. doi:10.1046/j.1365-313x.1998.00089.x
Other claims from this paper (19)
- Intervention effect Antisense expression of plastid aldolase decreased aldolase expression at the transcript and protein level in several potato transformants.
- Intervention effect Aldolase activity was decreased by 32-43% in transformant A-70.
- Intervention effect Aldolase activity was decreased by 56-71% in transformant A-3.
- Intervention effect Aldolase activity was decreased by 79-83% in transformant A-51.
- Intervention effect Aldolase activity was decreased by 91-97% in transformant A-2.
- Intervention effect The transformants showed a small increase in Rubisco activity.
- Intervention effect The transformants showed a small decrease in phosphoribulokinase activity and larger but subproportional decreases in sedoheptulose-1,7-biphosphatase and plastid fructose-1,6-bisphosphatase activity.
- Intervention effect The transformants contained increased triose phosphates.
- Intervention effect The transformants contained very low ribulose-1,5-bisphosphate and glycerate-3-phosphate.
- Intervention effect Thylakoid energization was increased in the transformants, with photosystem II more reduced.
- Intervention effect Starch synthesis was decreased by 16% in transformant A-70.
- Intervention effect Starch synthesis was decreased by 36% in transformant A-3.
- Intervention effect Sucrose synthesis was less strongly inhibited than starch synthesis in the transformants.
- Intervention effect Ambient photosynthesis was inhibited by 10% in transformant A-70.
- Intervention effect Ambient photosynthesis was inhibited by 40% in transformant A-3.
- Intervention effect Ambient photosynthesis was inhibited by 66% in transformant A-51.
- Intervention effect Ambient photosynthesis was inhibited by 85% in transformant A-2.
- Intervention effect Plant growth was not significantly altered in transformant A-70.
- Intervention effect Plant growth was decreased by 41% in transformant A-3 and was severely inhibited in plants with under 20% of wild-type aldolase activity.