Claim · model-written summary
Cryopreserved synthetic seeds revived and germinated, with 93.3% of somatic embryos differentiating into shoots.
- Taxon
- Swertia Chirayita
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Swertia chirayitat0 - Treatment
- 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3e0
- Outcome
- somatic embryos differentiated into shootse0
- Value
- 93.3%e0
Verbatim
e0“Cryopreserved synthetic seeds were successfully revived and germinated on MS media supplemented with 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3 in which 93.3% somatic embryos differentiated into shoots”
t0“Swertia Chirayita”
Title
Rolika Gupta; Hemant Sood Cryoletters 2022. doi:10.54680/fr22510110612
Other claims from this paper (5)
- Intervention effect A medium with 2,4-D, BAP and TDZ at the stated rates gave the highest callus induction.
- Observation Somatic embryos were recovered after 5 weeks on the same medium.
- Observation Cryopreserved embryo-derived shoots had similar amarogentin content to controls, at 4.72 ± 0.11 ug/mg.
- Observation Cryopreserved embryo-derived shoots had similar mangiferin content to controls, at 14.54 ± 0.05 ug/mg.
- Recommendation The protocol was proposed for multiplying and cryopreserving this endangered herb.