Claim · model-written summary
Insertion of alternating MET-ARG residues in the hypervariable region of Gy4 proglycinin was tolerated when the insertion was short.
- Taxon
- soybean
Glycine maxt0 - Treatment
- Insertion of alternating MET-ARG residues in predicted turn regions of a hypervariable sequence in Gy4 proglycinine5
- Outcome
- trimer assemblye5
- Direction
- toleratede5
- Qualifier
- when the string was shorte5
Not reportedComparator
Verbatim
t0“Engineering of soybean seed storage proteins”
e5“Insertion of alternating MET-ARG residues in predicted turn regions of a hypervariable sequence in Gy4 proglycinin were tolerated when the string was short but inhibited trimer assembly as it became longer”
Title
Engineering of soybean seed storage proteins
Craig D. Dickinson; L. Floener; Robert P. Evans; et al. Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) 1987
Other claims from this paper (8)
- Observation Gy4 and Gy5 proglycinins and the a-subunit of beta-conglycinin self-assembled into trimers.
- Observation Proglycinin Gy2 (group-1 subunit) did not self-assemble.
- Observation Proglycinin Gy2 assembled into mixed trimers in combination with Gy4 proglycinin.
- Observation No assembly was observed for preproglycinins Gyl and Gy4.
- Observation A Gy4 proglycinin lacking 27 amino acids in a highly conserved internal sequence showed no assembly.
- Intervention effect Longer insertion of alternating MET-ARG residues inhibited trimer assembly of Gy4 proglycinin.
- Observation Several different long deletions in the hypervariable region gave different levels of trimer assembly.
- Mechanism The different levels of trimer assembly may depend on the secondary structures of the regions being joined in the engineered subunits.
Rubus