By interacting organism › Bacteria › Spiroplasmataceae › Spiroplasma melliferum

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The assay discriminated against other phytopathogenic spiroplasmas, including S. kunkelii and S. melliferum.

Taxon: CitrusCitrust0
Interactor: S. kunkeliiSpiroplasma kunkeliie2pathogen
Second interactor: S. melliferumSpiroplasma melliferume2pathogen
Outcome: discrimination against other phytopathogenic spiroplasmase2
Comparator: other phytopathogenic spiroplasmas, including S. kunkelii and S. melliferume2

Verbatim

e2
“discrimination against other phytopathogenic spiroplasmas, including S. kunkelii and S. melliferum”
✓ located verbatim in the source · abstract, characters 1041–1139

t0
“Citrus Stubborn Disease”
✓ located verbatim in the source · title, characters 64–87

Title

Field-Compatible Detection of Spiroplasma citri Associated with Citrus Stubborn Disease Using CRISPR-Cas12a and Crude Sample Preparation

Justin Shih; Raymond K. Yokomi; Subhas Hajeri; et al. Plant Disease 2026. doi:10.1094/pdis-08-25-1699-re

Other claims from this paper (6)

Method / resource The fluorescence plate-reader DETECTR assay detected Spiroplasma citri at 1 aM.
Method / resource Detection limit with blue-light visualization and LFA was 10 aM for Spiroplasma citri.
Method / resource Plate-reader DETECTR results from symptomatic citrus DNA agreed 100% with qPCR.
Method / resource Crude extracts preserved full diagnostic sensitivity in fluorescence assays compared with kit-extracted samples.
Method / resource Crude extracts achieved 70% accuracy in LFA and visual formats in a subset of the samples.
Observation A 10-minute NaOH-Tris crude extraction protocol facilitated straightforward, equipment-free sample preparation.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sap from F. defendens-treated melon plants inhibited the growth of Spiroplasma melliferum.

Taxon: melonCucumis meloe0
Treatment: F. defendense1
Interactor: Spiroplasma melliferumSpiroplasma melliferume1other
Second interactor: F. defendensFrateuria defendense1biocontrol_agent
Outcome: growth of Spiroplasma melliferume1
Direction: inhibitede1

Not reportedComparator

Verbatim

e0
“The best isolate was introduced to melon plants and its cells were found in the plant sap 5 and 10 days post application”
✓ located verbatim in the source · abstract, characters 335–455

e1
“Sap, obtained from F. defendens- treated plants, inhibited the growth of Spiroplasma melliferum(a model mollicute) and altered the endophytic community of the plants”
✓ located verbatim in the source · abstract, characters 457–622

Title

Further study on the potential of Frateuria defendens as a biocontrol agent against phytoplasmas

Lilach Iasur-Kruh; Suzana Moussa; Vered Naor Phytopathogenic Mollicutes 2025. doi:10.5958/2249-4677.2025.00066.9

Other claims from this paper (3)

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The antimicrobial activity of the Frateuria defendens isolate was retained in melon sap and confirmed against Spiroplasma melliferum.

Taxon: melonCucumis meloe5
Organ / stage: plant sape2
Interactor: Spiroplasma melliferumSpiroplasma melliferume2other
Second interactor: Frateuria defendensFrateuria defendense1biocontrol_agent
Outcome: antimicrobial activitye2

Verbatim

e1
“introducing the endophytic bacterium Frateuria defendens”
✓ located verbatim in the source · abstract, characters 241–297

e2
“The antimicrobial activity of this isolate was retained in the plant sap and was confirmed in a biological test on Spiroplasma melliferum”
✓ located verbatim in the source · abstract, characters 688–825

e5
“on melon ( Cucumis melo ) as a model plant”
✓ located verbatim in the source · abstract, characters 359–401

Title

Unraveling the ecological impact of a bacterial biocontrol agent applied to melon plants: insights into phenotypic, biochemical, and microbiota changes affected by Frateuria defendens

Suzana Moussa; Vered Naor; Lilach Iasur Kruh European Journal of Plant Pathology 2025. doi:10.1007/s10658-025-03012-5

Other claims from this paper (2)

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

S-medium filtrates inhibited S. melliferum.

Taxon: periwinkle plantletst0
Treatment: filtrates prepared from a specific rich medium (S-medium)e0
Interactor: S. melliferumSpiroplasma melliferume0pathogen
Outcome: inhibition of S. melliferume0
Direction: inhibitione0

Not reportedComparator

Verbatim

e0
“filtrates prepared from a specific rich medium (S-medium) exhibiting beneficial activities, including the inhibition of S. melliferum and enhanced plant growth”
✓ located verbatim in the source · abstract, characters 1100–1259

t0
“phytoplasma-infected periwinkle plantlets”
✓ located verbatim in the source · abstract, characters 962–1003

Title

Evaluation of the biocontrol activity of Frateuria defendens-derived metabolites against mollicutes

Alaa Naama-Amar; Yoram Gerchman; Lilach Iasur Kruh; et al. Plant Signaling & Behavior 2022. doi:10.1080/15592324.2022.2070355

Other claims from this paper (3)

Intervention effect S-medium filtrates enhanced plant growth.
Method / resource SBP capsules made separation of bacterial cells from metabolites easier.
Method / resource Metabolites collected with SBP capsules retained inhibition of S. melliferum growth.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Metabolites collected with SBP capsules retained inhibition of S. melliferum growth.

Taxon: periwinkle plantletst0
Interactor: S. melliferumSpiroplasma melliferume0pathogen
Outcome: inhibit S. melliferum growthe0

Not reportedComparatorValue

Verbatim

e0
“retaining the ability of these metabolites to inhibit S. melliferum growth and to benefit the host plant”
✓ located verbatim in the source · abstract, characters 1666–1770

t0
“phytoplasma-infected periwinkle plantlets”
✓ located verbatim in the source · abstract, characters 962–1003

Title

Evaluation of the biocontrol activity of Frateuria defendens-derived metabolites against mollicutes

Alaa Naama-Amar; Yoram Gerchman; Lilach Iasur Kruh; et al. Plant Signaling & Behavior 2022. doi:10.1080/15592324.2022.2070355

Other claims from this paper (3)

Intervention effect S-medium filtrates inhibited S. melliferum.
Intervention effect S-medium filtrates enhanced plant growth.
Method / resource SBP capsules made separation of bacterial cells from metabolites easier.

Spiroplasma melliferum 5 claims

5 claims