Claim · model-written summary
Flock House virus, an insect-infecting virus, depends on the fermentation pathway for replication.
- Taxon
- plantsViridiplantaee0
- Interactor
- Flock House virusFlock House viruse5pathogen
- Outcome
- dependencee5
- Qualifier
- insect-infectinge5
Verbatim
e0“Depletion of Pdc1 in combination with deletion of the homologous PDC5 in yeast or knockdown of Pdc1 and Adh1 in plants reduced the efficiency of tombusvirus replication.”
e5“We also show the dependence of turnip crinkle virus, bamboo mosaic virus, tobacco mosaic virus and the insect-infecting Flock House virus on the fermentation pathway”
Title
Wenwu Lin; Yuyan Liu; Melissa Molho; et al. PLoS Pathogens 2019. doi:10.1371/journal.ppat.1008092
Other claims from this paper (7)
- Intervention effect Depletion of Pdc1 combined with deletion of PDC5 in yeast reduced the efficiency of tombusvirus replication in plants.
- Intervention effect Knockdown of Pdc1 and Adh1 in plants reduced the efficiency of tombusvirus replication.
- Observation Pdc1 and Adh1 fermentation enzymes were efficiently recruited into the viral replication compartment.
- Observation Complementation showed that enzymatically functional Pdc1 is required to support tombusvirus replication in plants.
- Observation Both Pdc1 and Adh1 are required for efficient ATP generation within the tombusvirus replication compartment, measured with an ATP biosensor.
- Observation In vitro reconstitution showed that Pdc1 has a pro-viral function in the assembly of the viral replicase and the activation of the viral p92 RdRp.
- Observation Turnip crinkle virus, bamboo mosaic virus and tobacco mosaic virus depend on the fermentation pathway for replication in plants.
Mycoplasma